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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM6 antibody
See all MCM6 products (5) ...
Description
Goat polyclonal to MCM6
Tested applications
WB, IHC-Frmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - MCM6 antibody (ab3730)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IHC-Fr: Use at an assay dependent concentration. (PubMed: 21980527)
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Binds to chromatin during G1 and detach from it during S phase.
Target information above from: UniProt accessionQ14566
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
see all
Database links
- Entrez Gene: 4175 Human
- Entrez Gene: 17219 Mouse
- Entrez Gene: 29685 Rat
- Omim: 601806 Human
- SwissProt: Q14566 Human
- SwissProt: P97311 Mouse
- SwissProt: Q62724 Rat
- Unigene: 444118 Human
see all
Anti-MCM6 antibody images:
Western blot - MCM6 antibody (ab3730)
Predicted band size : 115 kDa
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.
Detection: ECL.
References for Anti-MCM6 antibody (ab3730)
This product has been referenced in:
- Kim TMet al. Gene expression analysis reveals the cell cycle and kinetochore genes participating in ischemia reperfusion injury and early development in kidney. PLoS One 6:e25679 (2011). IHC-Fr; Rat.Read more (PubMed: 21980527) »
See 1 publication for this product
Publishing research using ab3730? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"