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Anti-MCM6 antibody (ab3730)

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Overview

Product name

Anti-MCM6 antibody
See all MCM6 products (5) ...

Description

Goat polyclonal to MCM6

Tested applications

WB, IHC-Frmore details

Cross reactivity

Reacts with

Rat, Human

Predicted to work with

Mouse

Immunogen

Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 6 encoded by exon 17.

Positive control

HeLa cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - MCM6 antibody (ab3730)Western blot - MCM6 antibody (ab3730) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/2000 - 1/10000.Det...Read more →

    WB: 1/2000 - 1/10000.Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).

  • ShowHide

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent concentration. (PubMed: 21980527)

Target

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Sequence similarities

Belongs to the MCM family.
Contains 1 MCM domain.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus. Binds to chromatin during G1 and detach from it during S phase.

Target information above from: UniProt accessionQ14566 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA replication licensing factor MCM6 antibody
  • MCG40308 antibody
  • Mcm6 antibody
  • MCM6 minichromosome maintenance deficient 6 antibody
  • MCM6_HUMAN antibody
  • Minichromosome maintenance complex component 6 antibody
  • Minichromosome maintenance deficient (mis5 S. pombe) 6 antibody
  • Minichromosome maintenance deficient 6 homolog antibody
  • Minichromosome maintenance protein 6 antibody
  • Mis5 antibody
  • MIS5 homolog antibody
  • P105MCM antibody
see all

Anti-MCM6 antibody images:

  Western blot - MCM6 antibody (ab3730)

Western blot - MCM6 antibody (ab3730)



Predicted band size : 115 kDa


Sample: Hela cell RIPA extract (60µg).

Antibody: Affinity purified Goat anti-MCM 6 (ab3730) diluted 1 / 4000.

Detection: ECL.

References for Anti-MCM6 antibody (ab3730)

This product has been referenced in:

  • Kim TMet al. Gene expression analysis reveals the cell cycle and kinetochore genes participating in ischemia reperfusion injury and early development in kidney. PLoS One 6:e25679 (2011). IHC-Fr; Rat.Read more (PubMed: 21980527) »

See 1 publication for this product

Publishing research using ab3730? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"