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Our end user want to obtain some reference (publish all valid customer reviews (valid means enough data and proof of purchase). Please send me some information about ab4461 or 4458.
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ANSWER: |
Thanks for your enquiry. Unfortunately, we are not aware of any publications that feature the use of either of these antibodies. We also have not received any feedback from customers in the form of reviews who have used these antibodies. For both ab4461 and ab4458, there is a nice Western image on the online datasheets. However, that is all the information that we have at this time. Please let me know if you have any additional questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 91 kDa
Sample: HeLa cell RIPA extract (50 µg).
Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.
IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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