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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM6 antibody
See all MCM6 products (5) ...
Description
Rabbit polyclonal to MCM6
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for or IP.
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Binds to chromatin during G1 and detach from it during S phase.
Target information above from: UniProt accessionQ14566
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DNA replication licensing factor MCM6 antibody
- MCG40308 antibody
- Mcm6 antibody
see all
Database links
- Entrez Gene: 4175 Human
- Entrez Gene: 17219 Mouse
- Entrez Gene: 29685 Rat
- Omim: 601806 Human
- SwissProt: Q14566 Human
- SwissProt: P97311 Mouse
- SwissProt: Q62724 Rat
- Unigene: 444118 Human
see all
Anti-MCM6 antibody images:
Western blot - MCM6 antibody (ab4458)
Predicted band size : 91 kDa
Sample: HeLa cell RIPA extract (50 µg).
Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MCM6 antibody(ab4458)
IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-MCM6 antibody (ab4458)
This product has been referenced in:
- Kawabata Tet al. A reduction of licensed origins reveals strain-specific replication dynamics in mice. Mamm Genome 22:506-17 (2011). WB; Mouse.Read more (PubMed: 21611832) »
- Takahashi Net al. The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. PLoS Genet 6:e1000817 (2010). WB; Human.Read more (PubMed: 20090939) »
See all 2 publications for this product
Publishing research using ab4458? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"