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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Anti-MCM6 antibody
See all MCM6 products (5) ...
Rabbit polyclonal to MCM6
WB, IHC-Pmore details
Reacts with
Mouse, Human
Predicted to work with
Rat
Synthetic peptide (Human) - 31 amino acids which represented a portion of human MCM 6 encoded by exon 17.
Liquid
Store at +4°C.
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Our Abpromise guarantee covers the use of ab4458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for or IP.
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Belongs to the MCM family.
Contains 1 MCM domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Binds to chromatin during G1 and detach from it during S phase.
Target information above from: UniProt accessionQ14566
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MCM6 antibody (ab4458)

Predicted band size : 91 kDa
Sample: HeLa cell RIPA extract (50 µg).
Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MCM6 antibody(ab4458)

IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab4458? Please let us know so that we can cite the reference in this datasheet
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Predicted band size : 91 kDa
Sample: HeLa cell RIPA extract (50 µg).
Antibody: ab4458 at 0.2 µg/ml incubated for 1 hour.

IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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