MCM7 antibody [47DC141] (ab2360)

Product Name 

MCM7 antibody [47DC141]

Product type 

Primary antibodies

Description 

Mouse monoclonal [47DC141] to MCM7

Immunogen 

Recombinant full length protein (Human).

Reacts with 
(species key)

Hu, Ms, Rat, Dog, Xl

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
WB: 1/25 - 1/50 for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation.
By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7.
IP: Use at 2µg/mg of lysate.
IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100 in an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

47DC141

Isotype 

IgG1

Purity 

IgG fraction

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C. Do not freeze.

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References for MCM7 antibody [47DC141] (ab2360)

  This product has been used in: (two most recent references)

Pillaire MJ et al. A 'DNA replication' signature of progression and negative outcome in colorectal cancer. Oncogene 29:876-87 (2010). IF; Human. PubMed: 19901968

van Dekken H et al. Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet 189:37-42 (2009). PubMed: 19167610

See all 4 publication references for this product.


If you publish research using ab2360 please let us know so that we can cite the reference on this datasheet.

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