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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM7 antibody
See all MCM7 products (10) ...
Description
Rabbit polyclonal to MCM7
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Rat, Human
Does not react with
Mouse
Immunogen
Synthetic peptide corresponding to residues near the N-terminus of human MCM7.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 0.87% Sodium chloride, 0.09% Tris glycine
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Western blot - MCM7 antibody (ab51062)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
- 1 Image
WB
Application notes
IP: 1/20.
WB: 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
Is unsuitable for ICC/IF or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP33993
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Database links
- Entrez Gene: 4176 Human
- Entrez Gene: 288532 Rat
- Omim: 600592 Human
- SwissProt: P33993 Human
- SwissProt: Q6AYN8 Rat
- Unigene: 438720 Human
Anti-MCM7 antibody images:
Western blot - MCM7 antibody (ab51062)
Anti-MCM7 antibody (ab51062) at 1/5000 dilution + HeLa cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size : 81 kDa
Observed band size : 81 kDa
References for Anti-MCM7 antibody (ab51062)
ab51062 has not yet been referenced specifically in any publications.
Publishing research using ab51062? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"