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Western blot - MCP1 antibody (ab25124)
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Product Name
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MCP1 antibody
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See all MCP1 antibodies (19)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MCP1
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Immunogen
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Recombinant fragment: GCAGAGACAC AGACAGAGGC CAGCCCAGAA ACCAGCCAAC TCTCACTGAA GCCAGATCTC TCTTCCTCCA corresponding to N terminal amino acids 1-73 of Rat MCP1
BLAST 'GCAGAGACAC AGACAGAGGC CAGCCCAGAA ACCAGCCAAC TCTCACTGAA GCCAGATCTC TCTTCCTCCA ' with  or 
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Reacts with
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Ms, Rat
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Specificity
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ab25124 reacts with both rat MCP1 and its mouse homolog JE.
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Tested applications
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Flow Cyt, Neut, WB
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Abreviews
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Application notes
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Recommended dilutions
Flow Cyt: Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells(see Abreview for further information).
Neut: Use at an assay dependent dilution.
WB: 1/2000. Detects a band of approximately 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
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Relevance
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This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MCP1 antibody images, references, products related to ab25124 and other tools.
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MCP1 antibody images:
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 Western blot - MCP1 antibody (ab25124)
All lanes : MCP1 antibody (ab25124) at 1/1000 dilution
Lane 1 : 40ug whole rat spinal cord protein lysate
Lane 2 : 40ug whole mouse spinal cord protein lysate
Lane 3 : Naive Rat
Lane 4 : Sham 1
Lane 5 : Sham 2
Lane 6 : Injured 1
Lane 7 : Injured 2
Lane 8 : Injured 3
Secondary
Goat anti-Rabbit HRP
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.
See Abreview
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Search PubMed (MEDLINE) for references to MCP1
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MCP1 antibody - more information
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Customer reviews (feedback) regarding MCP1 antibody |
Customer FAQs regarding MCP1 antibody |
Protocols for MCP1 antibody |
Price and availability of products related to MCP1 antibody |
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MCP1 antibody - related products:
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MCP1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about MCP1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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