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Products:Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
Overview
Product name
Anti-MCP1 antibody
See all MCP1 products (22) ...
Description
Rabbit polyclonal to MCP1
Specificity
ab25124 reacts with both rat MCP1 and its mouse homolog JE.
Tested applications
Flow Cyt, Neutralising, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Recombinant fragment: GCAGAGACACAGACAGAGGCCAGCCCAGAAACCAGCCAAC TCTCACTGAAGCCAGATCTCTCTTCCTCCACCA, corresponding to N terminal amino acids 1-73 of Rat MCP1
GCAGAGACAC AGACAGAGGC CAGCCCAGAA ACCAGCCAAC TCTCACTGAA GCCAGATCTC TCTTCCTCCA CCA
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
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Western blot - MCP1 antibody (ab25124)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt
Three stars (1 Abreview)Neutralising
Application notes
Flow Cyt: Used at a dulition of 1/100 for 30 min on rat primary mixed cortical cells(see Abreview for further information).
Neut: Use at an assay dependent dilution.
WB: 1/2000. Detects a band of approximately 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Post-translational
modifications
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Cellular localization
Secreted.
Target information above from: UniProt accessionP13500
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HSMCR30 antibody
- C-C motif chemokine 2 antibody
- CCL 2 antibody
see all
Database links
- Entrez Gene: 20296 Mouse
- Entrez Gene: 24770 Rat
- SwissProt: P10148 Mouse
- SwissProt: P14844 Rat
- Unigene: 290320 Mouse
- Unigene: 4772 Rat
Anti-MCP1 antibody images:
Western blot - MCP1 antibody (ab25124)
All lanes : Anti-MCP1 antibody (ab25124) at 1/1000 dilution
Lane 1 : 40ug whole rat spinal cord protein lysate
Lane 2 : 40ug whole mouse spinal cord protein lysate
Lane 3 : Naive Rat
Lane 4 : Sham 1
Lane 5 : Sham 2
Lane 6 : Injured 1
Lane 7 : Injured 2
Lane 8 : Injured 3
Secondary
Goat anti-Rabbit HRP
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 2 minutes
This image is courtesy of an Abreview by Nancy Nutile-McMenemy submitted on 7 December 2005.
References for Anti-MCP1 antibody (ab25124)
This product has been referenced in:
- Pechenino ASet al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB; Rat.Read more (PubMed: 21750230) »
See 1 publication for this product
Publishing research using ab25124? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"