Overview

  • Product nameAnti-MCP1 antibody
    See all MCP1 primary antibodies
  • Description
    Rabbit polyclonal to MCP1
  • Tested applicationsIHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Rat).

  • Positive control
    • RAW 264.7 cells (mouse macrophage/monocyte adherent cell line) stimulated with LPS for 15 hours

Properties

Applications

Our Abpromise guarantee covers the use of ab7202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution. PubMed: 21088249
ICC/IF Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 18 kDa.

Target

  • FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-C motif chemokine 2 antibody
    • CCL 2 antibody
    • CCL2 antibody
    • CCL2_HUMAN antibody
    • Chemokine (C C motif) ligand 2 antibody
    • Chemokine C C motif ligand 2 antibody
    • Chemokine CC Motif Ligand 2 antibody
    • GDCF 2 antibody
    • GDCF 2 HC11 antibody
    • GDCF-2 antibody
    • GDCF2 antibody
    • HC11 antibody
    • HSMCR30 antibody
    • HSMCR30 antibody
    • JE antibody
    • MCAF antibody
    • MCP 1 antibody
    • MCP-1 antibody
    • MCP1 antibody
    • MGC9434 antibody
    • Monocyte chemoattractant protein 1 antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic and activating factor antibody
    • Monocyte chemotactic protein 1 antibody
    • Monocyte secretory protein JE antibody
    • Monocyte secretory protein JE antibody
    • SCYA2 antibody
    • Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibody
    • Small inducible cytokine A2 antibody
    • Small inducible cytokine subfamily A (Cys Cys), member 2 antibody
    • Small inducible cytokine subfamily A Cys Cys member 2 antibody
    • Small-inducible cytokine A2 antibody
    • SMC CF antibody
    • SMC-CF antibody
    • SMCCF antibody
    see all

Anti-MCP1 antibody images

References for Anti-MCP1 antibody (ab7202)

This product has been referenced in:
  • Chew SH  et al. Cancer-promoting role of adipocytes in asbestos-induced mesothelial carcinogenesis through dysregulated adipocytokine production. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 23917077) »
  • Ataka K  et al. Bone marrow-derived microglia infiltrate into the paraventricular nucleus of chronic psychological stress-loaded mice. PLoS One 8:e81744 (2013). IHC-Fr ; Mouse . Read more (PubMed: 24303068) »

See all 18 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (liver)
Loading amount 30 µg
Specification liver
Treatment 2mg/kg LPS 1h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 16 2012

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your note.



This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab7202. For your information, the internal reference note for...

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Thank you for your enquiry regarding ab7202 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

The protocol looks fine to me. It may wel...

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Thank you for your phone call today, and I apologize for any miscommunication with my colleague yesterday. Your credit note ID for ab20641 is: ***. Your credit note ID for ab7202 is: ***. I am sorry that these antibodies did not perform as stated on th...

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Thank you for your call today and for your questions about ab7202. As we discussed, we recommend using a microwave antigen retrieval method with this antibody. However, if the antibody is still staining the wrong localization, please let me know a...

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Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (injred spinal cord tissue)
Specification injred spinal cord tissue
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. soo ryung jeong

Verified customer

Submitted Oct 01 2009

I'm sorry to hear the customer is having problems with his new vial of ab7202. Thank you for taking the time to fill in the questionnaire, I can see clearly that the protocol looks very good and the antibody should perform if the customer has previousl...

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I'm sorry to hear you are having problem with ab7102. We have not tested it in mouse aorta or have any reviews yet from customers using it in aorta. So please do let us know when your staining works and we will reward you with an Amazon gift voucher (a...

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Thank you for your phone call. There are no special conditions that need to be followed to use ab7202 in IHC-frozen sections and so I suggest fixing your sections with acetone as per our general IHC protocol for frozen sections (the protocol can be fou...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"