Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
Overview
Product name
Anti-MCP1 antibody
See all MCP1 products (22) ...
Description
Rabbit polyclonal to MCP1
Tested applications
IHC-P, IHC-Fr, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Recombinant full length protein (Rat).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
-
Western blot - MCP1 antibody (ab7202)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21088249)
ICC/IF: Use at an assay dependent dilution.
WB: 1/2000Predicted molecular weight: 18 kDa.
Target
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Post-translational
modifications
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Cellular localization
Secreted.
Target information above from: UniProt accessionP13500
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HSMCR30 antibody
- C-C motif chemokine 2 antibody
- CCL 2 antibody
see all
Database links
- Entrez Gene: 20296 Mouse
- Entrez Gene: 24770 Rat
- SwissProt: P10148 Mouse
- SwissProt: P14844 Rat
- Unigene: 290320 Mouse
- Unigene: 4772 Rat
Anti-MCP1 antibody images:
Western blot - MCP1 antibody (ab7202)
Predicted band size : 18 kDa
Observed band size : 12 kDa (why is the actual band size different from the predicted?)
Additional bands at : 18 kDa (possible dimer).
References for Anti-MCP1 antibody (ab7202)
This product has been referenced in:
- Kanellakis Pet al. High-mobility group box protein 1 neutralization reduces development of diet-induced atherosclerosis in apolipoprotein e-deficient mice. Arterioscler Thromb Vasc Biol 31:313-9 (2011). IHC-Fr; Mouse.Read more (PubMed: 21088249) »
- Jo SMet al. Up-regulation of endothelial endothelin-1 expression prior to vasogenic edema formation in the rat piriform cortex following status epilepticus. Neurosci Lett 501:25-30 (2011).Read more (PubMed: 21742016) »
See all 12 publications for this product
Publishing research using ab7202? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"