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Products:Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
Overview
Product name
Anti-MCP1 antibody
See all MCP1 products (22) ...
Description
Rabbit polyclonal to MCP1
Specificity
The antibody reacts with human MCP-1 both natural and recombinant. The antigen specificity was assessed by ELISA. The antigen specificity was further assessed by immunoprecipitation and Western blot. No cross-reactivities with other cytokines have been detected.
Tested applications
Neutralising, WB, Inhibition Assay, IHC-Fr, ICCmore details
Cross reactivity
Reacts with
Human, Pig
General notes
Human monocyte chemotactic protein-1 (MCP-1) is also known as MCAF.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
0.2 µm filtered
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Neut: Use at an assay dependent dilution. (For neutralization of biological activity in vitro dilutions have to be made according to the amount of MCP-1 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.)
WB: 1/10Predicted molecular weight: 11.9 kDa.
Inhibition Assay
Inhib: 1/10Inhib: 1/10
IHC-Fr
IHC-Fr: 1/10IHC-Fr: 1/10
ICC
ICC: 1/10ICC: 1/10
Target
Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Post-translational
modifications
Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
Cellular localization
Secreted.
Target information above from: UniProt accessionP13500
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HSMCR30 antibody
- C-C motif chemokine 2 antibody
- CCL 2 antibody
see all
Database links
- Entrez Gene: 6347 Human
- Entrez Gene: 397422 Pig
- Omim: 158105 Human
- SwissProt: P13500 Human
- SwissProt: P42831 Pig
- Unigene: 303649 Human
References for Anti-MCP1 antibody (ab7814)
This product has been referenced in:
- Zakkar Met al. Dexamethasone arterializes venous endothelial cells by inducing mitogen-activated protein kinase phosphatase-1: a novel antiinflammatory treatment for vein grafts? Circulation 123:524-32 (2011). IF, IHC; Pig.Read more (PubMed: 21262999) »
- Hubal MJet al. Inflammatory gene changes associated with the repeated-bout effect. Am J Physiol Regul Integr Comp Physiol 294:R1628-37 (2008). IHC-Fr; Human.Read more (PubMed: 18353886) »
See all 2 publications for this product
Publishing research using ab7814? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"