|
|
|
|
|
Product Name
|
MCP1 antibody
|
| |
See all MCP1 antibodies (19)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to MCP1
|
|
Reacts with
(species key)
|
Hu
|
|
Specificity
|
The antibody reacts with human MCP-1 both natural and recombinant. The antigen specificity was assessed by ELISA. The antigen specificity was further assessed by immunoprecipitation and Western blot. No cross-reactivities with other cytokines have been detected.
|
|
Tested applications
(see key)
|
ICC, IHC-Fr, Inhib, Neut, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC: 1/10.
IHC-Fr: 1/10 (see Yu et al).
Inhib: 1/10.
Neut: Use at an assay dependant dilution.
WB: 1/10. Predicted molecular weight: 11.9 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
For neutralization of biological activity in vitro dilutions have to be made according to the amount of MCP-1 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Response to hypoxia
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Alpha Chemokines (CXC)
|
|
Relevance
|
This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 0.1% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
0.2 µm filtered
|
|
Form
|
Liquid
|
|
Concentration
|
0.100 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
Human monocyte chemotactic protein-1 (MCP-1) is also known as MCAF.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MCP1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for MCP1 antibody (ab7814)
|
|
This product has been used in: (two most recent references)
Hubal MJ et al. Inflammatory gene changes associated with the repeated-bout effect. Am J Physiol Regul Integr Comp Physiol 294:R1628-37 (2008). IHC-Fr; Human. PubMed: 18353886
Yu X et al. Expression of monocyte chemoattractant protein-1 in delayed type hypersensitivity reactions in the skin. Lab Invest 71:226-35 (1994). PubMed: 8078302
If you publish research using ab7814 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to MCP1
|
| |
MCP1 antibody - more information
|
Customer reviews (feedback) regarding MCP1 antibody |
Customer FAQs regarding MCP1 antibody |
Protocols for MCP1 antibody |
Price and availability of products related to MCP1 antibody |
| |
MCP1 antibody - related products:
|
|
|
| |
MCP1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about MCP1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
