Anti-MCP1 antibody (ab9669)
- Product nameAnti-MCP1 antibodySee all MCP1 primary antibodies ...
- DescriptionRabbit polyclonal to MCP1
- Tested applicationsWB, ELISA, Neutralising, IHC-P, ICC/IF, Sandwich ELISA more details
- Species reactivityReacts with: Human
Highly pure (>98%) recombinant hMCAF/MCP-1 (human Macrophage/Monocyte chemotactic protein-1)
- FormLyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Light chain typeunknown
Our Abpromise guarantee covers the use of ab9669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 0.1 - 0.2 µg/ml. To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCAF/MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||ELISA: Use a concentration of 0.5 µg/ml. To detect hMCAF/MCP-1 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMCAF/MCP-1.|
|Neutralising||Neut: Use a concentration of 3 - 5 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of hMCAF/MCP-1 (10.0 ng/ml), a concentration of 3.0 - 5.0 µg/ml of this antibody is required.|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: Use at an assay dependent dilution. PubMed: 20369226|
|Sandwich ELISA||sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to MCP1 (ab9858). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab9868 as Capture.|
- FunctionChemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
- Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
- Cellular localizationSecreted.
- C-C motif chemokine 2 antibodyCCL 2 antibodyCCL2 antibody
- CCL2_HUMAN antibodyChemokine (C C motif) ligand 2 antibodyChemokine C C motif ligand 2 antibodyChemokine CC Motif Ligand 2 antibodyGDCF 2 antibodyGDCF 2 HC11 antibodyGDCF-2 antibodyGDCF2 antibodyHC11 antibodyHSMCR30 antibodyHSMCR30 antibodyJE antibodyMCAF antibodyMCP 1 antibodyMCP-1 antibodyMCP1 antibodyMGC9434 antibodyMonocyte chemoattractant protein 1 antibodyMonocyte chemotactic and activating factor antibodyMonocyte chemotactic and activating factor antibodyMonocyte chemotactic protein 1 antibodyMonocyte secretory protein JE antibodyMonocyte secretory protein JE antibodySCYA2 antibodySmall inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je) antibodySmall inducible cytokine A2 antibodySmall inducible cytokine subfamily A (Cys Cys), member 2 antibodySmall inducible cytokine subfamily A Cys Cys member 2 antibodySmall-inducible cytokine A2 antibodySMC CF antibodySMC-CF antibodySMCCF antibody
Anti-MCP1 antibody images
ab9669 at 2.5 µg/ml staining human breast invasive ductal carcinoma tissue by IHC-P. The anitbody was incubated for two hours at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended.
HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 µM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Anti-MCP1 antibody (ab9669) at 1/4000 dilution + lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/20000 dilution
developed using the ECL technique
Observed band size : 12-14 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
Image courtesy of Dr Laura Toma by Abreview.
All lanes : Anti-MCP1 antibody (ab9669) at 0.2 µg/ml
Lane 1 :
MCP1 protein (Active) (ab73866) at 0.01 µg
Lane 2 :
MCP1 protein (Active) (ab73866) at 0.001 µg
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-MCP1 antibody (ab9669)
This product has been referenced in:
- Tsuyada A et al. CCL2 mediates cross-talk between cancer cells and stromal fibroblasts that regulates breast cancer stem cells. Cancer Res 72:2768-79 (2012). Read more (PubMed: 22472119) »
- Toma L et al. Anti-oxidant and anti-inflammatory mechanisms of amlodipine action to improve endothelial cell dysfunction induced by irreversibly glycated LDL. Biochem Biophys Res Commun 411:202-7 (2011). WB ; Human . Read more (PubMed: 21729693) »