You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameMCRS1 protein (Tagged-His Tag)See all MCRS1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 14-317 (304 amino acids) of Human MCRS1 with N terminal His tag; MWt 35 kDa (UniProt Q96EZ8).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 61 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesMASGTASRSEDEESLAGQKRASSQALGTIPKRRSSSRFIK RKKFDDELV ESSLAKSSTRAKGASGVEPGRCSGSEPSS SEKKKVSKAPSTPVPPSPAP APGLTKRVKKSKQPLQVT KDLGRWKPADDLLLINAVLQTNDLTSVHLGV KFSCRFT LREVQERWYALLYDPVISKLACQAMRQLHPEAIAAIQSKA LF SKAEEQLLSKVGSTSQPTLETFQDLLHRHPDAFYLA RTAKALQAHWQLM KQYYLLEDQTVQPLPKGDQVLNFSD AEDLIDDSKLKDMRDEVLEHELMV ADRRQKREIR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92134 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        58 kDa microspherule proteinCell cycle regulated factor (78 kDa)Cell cycle regulated factor p78
        Cell cycle-regulated factor p78ICP22BPINO80 complex subunit JMCRS 1MCRS 2MCRS1MCRS1_HUMANMCRS2Microspherule protein 1MSP 58MSP58p78
      see all
  • FunctionModulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. May be an inhibitor of TERT telomerase activity.
    Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
  • Tissue specificityDetected in testis, and at lower levels in spleen, thymus, prostate, uterus, small intestine, colon and leukocytes.
  • Sequence similaritiesContains 1 FHA domain.
  • Developmental stageCell-cycle regulated: levels are highest early in S phase; not detectable in G2.
  • Cellular localizationNucleus. Nucleus > nucleolus. In microspherules in the nucleolus.

    • Target information above from: UniProt accession Q96EZ8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    MCRS1 protein (Tagged-His Tag) images

    • SDS-PAGE - MCRS1 protein (Tagged-His Tag) (ab92134)
      SDS-PAGE - MCRS1 protein (Tagged-His Tag) (ab92134)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for MCRS1 protein (Tagged-His Tag) (ab92134)

    ab92134 has not yet been referenced specifically in any publications.

    Publishing research using ab92134 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab92134 Submit a Question for ab92134

    There are currently no Abreviews or Questions for ab92134.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"