![Sandwich ELISA - MCSF antibody [21] (ab18645) image](#MCSF-Primary-antibodies-ab18645-4.jpg)
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Product name
Anti-MCSF antibody [21]
See all MCSF products (14) ...
Description
Mouse monoclonal [21] to MCSF
Specificity
ab18645 detects rhM-CSF. ab18645 does not cross react with other human cytokines tested such as IL1 beta, IL8, IL16, EGF,G-CSFR, GM-CSF, MCP1, MCP3, TGF beta and TNF alpha.
Tested applications
ELISA, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified recombinant MCSF (Human).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.01M PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
21
Myeloma
Sp2/0-Ag14
Isotype
IgG2a
Light chain type
kappa
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> CSFs
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Sandwich ELISA - MCSF antibody [21] (ab18645)
![Sandwich ELISA - MCSF antibody [21] (ab18645) image](/ps/datasheet/images/18/ab18645/MCSF-Primary-antibodies-ab18645-4.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab18645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
sELISA: Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MCSF (ab9693). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab9693 as Detection.
Target
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Post-translational
modifications
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localization
Cell membrane and Secreted > extracellular space.
Target information above from: UniProt accessionP09603
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
Database links
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
Anti-MCSF antibody [21] images:
Sandwich ELISA - MCSF antibody [21] (ab18645)
Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.
Protocols
References for Anti-MCSF antibody [21] (ab18645)
ab18645 has not yet been referenced specifically in any publications.
Publishing research using ab18645? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"