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Overview

  • Product nameAnti-MCSF antibody [EP1179Y]See all MCSF primary antibodies ...
  • Description
    Rabbit monoclonal [EP1179Y] to MCSF
  • Tested applicationsWB, IP, IHC-P, ICC more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues on human MCSF.

  • Positive controlWB: Recombinant MCSF IHC-P: Human tonsil tissue.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab52864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
IHC-P
ICC
  • Application notesICC: 1/250 - 1/500.
    IHC-P: 1/50 - 1/100. Antigen retrieval is recommended.
    IP: 1/50.
    WB: 1/50000. Predicted molecular weight: 60 kDa.

    Is unsuitable for Flow Cyt.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
    • Post-translational
      modifications      Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
      Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
    • Cellular localizationCell membrane and Secreted > extracellular space.

      • Target information above from: UniProt accession P09603 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          Colony stimulating factor 1 (macrophage) antibodyColony stimulating factor 1 antibodyColony stimulating factor macrophage specific antibody
          CSF 1 antibodyCSF-1 antibodyCSF1 antibodyCSF1_HUMAN antibodyCsfm antibodyLanimostim antibodyM CSF antibodyM-CSF antibodyMacrophage Colony Stimulating Factor 1 antibodyMacrophage colony stimulating factor antibodyMCSF antibodyMGC31930 antibodyProcessed macrophage colony-stimulating factor 1 antibody
        see all

      Anti-MCSF antibody [EP1179Y] images

      • Western blot - MCSF antibody [EP1179Y] (ab52864)
        Western blot - MCSF antibody [EP1179Y] (ab52864)
        Anti-MCSF antibody [EP1179Y] (ab52864) at 1/50000 dilution + recombinant MCSF at 0.005 µg

        Secondary
        Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size : 60 kDa
        Observed band size : 20 kDa (why is the actual band size different from the predicted?)
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCSF antibody [EP1179Y] (ab52864)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCSF antibody [EP1179Y] (ab52864)
        ab52864 at 1/50 dilution stainng MCSF in human tonsil by Immunohistochemistry, Paraffin embedded tissue.

      References for Anti-MCSF antibody [EP1179Y] (ab52864)

      This product has been referenced in:
      • Taylor RM  et al. VEGF, FLT3 ligand, PlGF and HGF can substitute for M-CSF to induce human osteoclast formation: implications for giant cell tumour pathobiology. Lab Invest 92:1398-406 (2012). WB . Read more (PubMed: 22906984) »
      • Taylor RM  et al. Analysis of stromal cells in osteofibrous dysplasia and adamantinoma of long bones. Mod Pathol 25:56-64 (2012). Read more (PubMed: 21983933) »

      See all 3 Publications for this product

      Publishing research using ab52864 ? Please let us know so that we can cite the reference in this datasheet

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