Overview

  • Product nameAnti-MCSF antibody [EP1179Y]
    See all MCSF primary antibodies
  • Description
    Rabbit monoclonal [EP1179Y] to MCSF
  • Tested applicationsWB, IP, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Cow, Chimpanzee
  • Immunogen

    A synthetic peptide corresponding to residues on human MCSF.

  • Positive control
    • WB: Recombinant MCSF IHC-P: Human tonsil tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab52864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC
  • Application notesICC: 1/250 - 1/500.
    IHC-P: 1/50 - 1/100. Antigen retrieval is recommended.
    IP: 1/50.
    WB: 1/50000. Predicted molecular weight: 60 kDa.

    Is unsuitable for Flow Cyt.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGranulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
    • Post-translational
      modifications
      Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
      Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
    • Cellular localizationCell membrane and Secreted > extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Colony stimulating factor 1 (macrophage) antibody
      • Colony stimulating factor 1 antibody
      • Colony stimulating factor macrophage specific antibody
      • CSF 1 antibody
      • CSF-1 antibody
      • CSF1 antibody
      • CSF1_HUMAN antibody
      • Csfm antibody
      • Lanimostim antibody
      • M CSF antibody
      • M-CSF antibody
      • Macrophage Colony Stimulating Factor 1 antibody
      • Macrophage colony stimulating factor antibody
      • MCSF antibody
      • MGC31930 antibody
      • Processed macrophage colony-stimulating factor 1 antibody
      see all

    Anti-MCSF antibody [EP1179Y] images

    • ab52864 at 1/50 dilution stainng MCSF in human tonsil by Immunohistochemistry, Paraffin embedded tissue.
    • Anti-MCSF antibody [EP1179Y] (ab52864) at 1/50000 dilution + recombinant MCSF at 0.005 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 60 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)

    References for Anti-MCSF antibody [EP1179Y] (ab52864)

    This product has been referenced in:
    • Wang YW  et al. Clinicopathological significance of microRNA-214 in gastric cancer and its effect on cell biological behaviour. PLoS One 9:e91307 (2014). WB ; Human . Read more (PubMed: 24614175) »
    • Behnes CL  et al. Tumor-associated macrophages are involved in tumor progression in papillary renal cell carcinoma. Virchows Arch 464:191-6 (2014). Read more (PubMed: 24327306) »

    See all 11 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"