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Products:Immunology >> Innate Immunity >> Cytokines >> CSFs
Overview
Product name
Anti-MCSF antibody
See all MCSF products (13) ...
Description
Mouse monoclonal to MCSF
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow, Dog, Chimpanzee, Rhesus monkey
Immunogen
Recombinant fragment with tag: EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK, corresponding to amino acids 33-132 of Human MCSF
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK
Positive control
MCSF transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> CSFs
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Western blot - MCSF antibody (ab66236)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 75 kDa (predicted molecular weight: 60 kDa).
Target
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Post-translational
modifications
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localization
Cell membrane and Secreted > extracellular space.
Target information above from: UniProt accessionP09603
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
Database links
- Entrez Gene: 281094 Cow
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
Anti-MCSF antibody images:
Western blot - MCSF antibody (ab66236)
All lanes : Anti-MCSF antibody (ab66236) at 1 µg/ml
Lane 1 : MCSF transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 60 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
References for Anti-MCSF antibody (ab66236)
ab66236 has not yet been referenced specifically in any publications.
Publishing research using ab66236? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"