Anti-MCSF antibody (ab9693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCSF antibody (ab9693)

ab9693 staining MCSF in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCSF antibody (ab9693)

ab9693 staining MCSF in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Western blot - Anti-MCSF antibody (ab9693)

Anti-MCSF antibody (ab9693) at 0.2 µg/ml + MCSF protein (ab9694) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 1 minute

Western blot - MCSF antibody (ab9693)

All lanes : Anti-MCSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)

Lane 1 : Human Kidney whole cell lysate
Lane 2 : Human Kidney whole cell lysate transfected with LFABP

Lysates/proteins at 15 µg per lane.

Secondary
An IRDye^®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution

Performed under non-reducing conditions.

Observed band size : 70 kDa (why is the actual band size different from the predicted?)


Blocking Step: 5% BSA for 1 hour at 25°C

Deatection method: Odyssey for 50 seconds.

Right-hand Image: Weaker exposure to identify bands.

This image is courtesy of an Abreview submitted by Valentina Marcelli

Sandwich ELISA - MCSF antibody (ab9693)

Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.