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Products:Immunology >> Innate Immunity >> Cytokines >> CSFs
Overview
Product name
Anti-MCSF antibody
See all MCSF products (13) ...
Description
Rabbit polyclonal to MCSF
Tested applications
WB, ELISA, Neutralising, Sandwich ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Highly pure (>98%) recombinant hM-CSF (human M-CSF)
Properties
Form
Lyophilised:
Reconstitute with 200µl of sterile water.
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Immunology >> Innate Immunity >> Cytokines >> CSFs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. (To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.)
ELISA: Use at an assay dependent dilution. (To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF.)
Neut: Use at an assay dependent dilution. (To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required.)
sELISA: Use a concentration of 0.1 µg/ml Can be paired for Sandwich ELISA with Mouse monoclonal [21] to MCSF (ab18645). (For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with Ab18645 as Capture.)
IHC-P: Use a concentration of 5 µg/ml
Target
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Post-translational
modifications
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
Cellular localization
Cell membrane and Secreted > extracellular space.
Target information above from: UniProt accessionP09603
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Colony stimulating factor 1 (macrophage) antibody
- Colony stimulating factor 1 antibody
- Colony stimulating factor macrophage specific antibody
see all
Database links
- Entrez Gene: 1435 Human
- Omim: 120420 Human
- SwissProt: P09603 Human
- Unigene: 591402 Human
Anti-MCSF antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCSF antibody (ab9693)
ab9693 staining MCSF in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCSF antibody (ab9693)
ab9693 staining MCSF in human cervical squamous cell carcinoma section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
Western blot - Anti-MCSF antibody (ab9693)
Anti-MCSF antibody (ab9693) at 0.2 µg/ml + MCSF protein (ab9694) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Western blot - MCSF antibody (ab9693)
All lanes : Anti-MCSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)
Lane 1 : Human Kidney whole cell lysate
Lane 2 : Human Kidney whole cell lysate transfected with LFABP
Lysates/proteins at 15 µg per lane.
Secondary
An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution
Performed under non-reducing conditions.
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Blocking Step: 5% BSA for 1 hour at 25°C
Deatection method: Odyssey for 50 seconds.
Right-hand Image: Weaker exposure to identify bands.
This image is courtesy of an Abreview submitted by Valentina Marcelli
Sandwich ELISA - MCSF antibody (ab9693)
Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.
Protocols
References for Anti-MCSF antibody (ab9693)
This product has been referenced in:
- Brooks PJet al. M-CSF accelerates orthodontic tooth movement by targeting preosteoclasts in mice. Angle Orthod 81:277-83 (2011). IHC-P; Mouse.Read more (PubMed: 21208080) »
- Chase Aet al. Imatinib sensitivity as a consequence of a CSF1R-Y571D mutation and CSF1/CSF1R signaling abnormalities in the cell line GDM1. Leukemia 23:358-64 (2009).Read more (PubMed: 18971950) »
See all 2 publications for this product
Publishing research using ab9693? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"