Recombinant Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13597(B)] to MDH1 - C-terminal
- Suitable for: Flow Cyt (Intra), ICC/IF, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MDH1 antibody [EPR13597(B)] - C-terminal
See all MDH1 primary antibodies -
Description
Rabbit monoclonal [EPR13597(B)] to MDH1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa and HepG2 whole cell lysate (ab7900); HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13597(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/250.
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IP |
1/50.
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WB |
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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Notes |
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Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/250. |
IP
1/50. |
WB
1/10000 - 1/50000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Target
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Sequence similarities
Belongs to the LDH/MDH superfamily. MDH type 2 family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4190 Human
- Entrez Gene: 17449 Mouse
- Entrez Gene: 24551 Rat
- Omim: 154200 Human
- SwissProt: P40925 Human
- SwissProt: P14152 Mouse
- SwissProt: O88989 Rat
- Unigene: 526521 Human
see all -
Alternative names
- cytoplasmic antibody
- Cytosolic malate dehydrogenase antibody
- Diiodophenylpyruvate reductase antibody
see all
Images
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All lanes : Anti-MDH1 antibody [EPR13597(B)] - C-terminal (ab180152) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/250 dilution. Counter stained with DAPI.
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Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (10)
ab180152 has been referenced in 10 publications.
- Marsilio S et al. Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma. J Vet Intern Med 35:179-189 (2021). PubMed: 33471936
- Ying M et al. Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions. Redox Biol 46:102065 (2021). PubMed: 34293554
- Nelson BS et al. Tissue of origin dictates GOT1 dependence and confers synthetic lethality to radiotherapy. Cancer Metab 8:1 (2020). PubMed: 31908776
- Labuschagne CF et al. Cell Clustering Promotes a Metabolic Switch that Supports Metastatic Colonization. Cell Metab 30:720-734.e5 (2019). PubMed: 31447323
- Zhang W et al. Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle. Bosn J Basic Med Sci N/A:N/A (2019). PubMed: 31215856
- Tajan M et al. A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3. Cell Metab 28:721-736.e6 (2018). PubMed: 30122553
- Gaude E et al. NADH Shuttling Couples Cytosolic Reductive Carboxylation of Glutamine with Glycolysis in Cells with Mitochondrial Dysfunction. Mol Cell 69:581-593.e7 (2018). PubMed: 29452638
- Bao Y et al. Carnosine Inhibits the Proliferation of Human Cervical Gland Carcinoma Cells Through Inhibiting Both Mitochondrial Bioenergetics and Glycolysis Pathways and Retarding Cell Cycle Progression. Integr Cancer Ther 17:80-91 (2018). PubMed: 28008780
- Pace MC et al. Changes in proteome solubility indicate widespread proteostatic disruption in mouse models of neurodegenerative disease. Acta Neuropathol 136:919-938 (2018). PubMed: 30140941
- Hanse EA et al. Cytosolic malate dehydrogenase activity helps support glycolysis in actively proliferating cells and cancer. Oncogene 36:3915-3924 (2017). PubMed: 28263970