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Western blot - ME1 antibody (ab55537)
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Product Name
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ME1 antibody
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See all ME1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to ME1
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Immunogen
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Recombinant full length protein, corresponding to amino acids 1-573 of Human ME1
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cell Biology >> Other Antibodies >> Oxidative Stress
Signal Transduction >> Metabolism >> Energy Metabolism
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Relevance
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ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.50 - 1.00 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ME1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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ME1 antibody images:
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 Western blot - ME1 antibody (ab55537)
Western blot against tagged recombinant protein immunogen using ab55537 ME1 antibody at 1ug/ml. Predicted band size of immunogen is 89 kDa
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References for ME1 antibody (ab55537)
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This product has been used in:
Pound KM et al. Substrate-enzyme competition attenuates upregulated anaplerotic flux through malic enzyme in hypertrophied rat heart and restores triacylglyceride content: attenuating upregulated anaplerosis in hypertrophy. Circ Res 104:805-12 (2009). WB. PubMed: 19213957
If you publish research using ab55537 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ME1
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ME1 antibody - more information
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Customer reviews (feedback) regarding ME1 antibody |
Customer FAQs regarding ME1 antibody |
Protocols for ME1 antibody |
Price and availability of products related to ME1 antibody |
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ME1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ME1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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