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Overview

  • Product nameAnti-ME2 antibodySee all ME2 primary antibodies ...
  • Description
    Rabbit polyclonal to ME2
  • Tested applicationsWB, ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human ME2.

  • Positive controlHuman placenta tissue. Extracts from LOVO cells and RAW264.7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab74122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 65 kDa.
ELISA ELISA: 1/40000.
IHC-P IHC-P: 1/50 - 1/100.

Target

  • Alternative names
      Malate dehydrogenase antibodyMalic enzyme 2 antibodyMalic enzyme 2 mitochondrial antibody
      Malic enzyme 2 NAD(+) dependent mitochondrial antibodyMalic enzyme mitochondrial antibodyMalic enzyme NAD(+) dependent mitochondrial antibodyMAOM_HUMAN antibodyME 2 antibodyME2 antibodymitochondrial antibodyNAD dependent malic enzyme mitochondrial antibodyNAD ME antibodyNAD-dependent malic enzyme antibodyNAD-ME antibodyODS1 antibodyPyruvic malic carboxylase antibody
    see all

Anti-ME2 antibody images

  • Western blot - ME2 antibody (ab74122)
    Western blot - ME2 antibody (ab74122)
    All lanes : Anti-ME2 antibody (ab74122) at 1/500 dilution

    Lane 1 : Extracts from LOVO cells
    Lane 2 : Extracts from RAW264.7 cells
    Lane 3 : Extracts from RAW264.7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ME2 antibody (ab74122)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ME2 antibody (ab74122)
    Immunohistochemical analysis of paraffin embedded human placenta tissue using ab74122, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

References for Anti-ME2 antibody (ab74122)

ab74122 has not yet been referenced specifically in any publications.

Publishing research using ab74122 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab74122 Submit a Question for ab74122

Displaying 1 - 4 of 4 results for Abreviews and Q&A

10
Votes: Highest First

I have checked the antibody you mentioned. I need an antibody which could recognize both mouse and human ME2, but ab112953 has not been confirmed for human ME2. I hope could get the refund from you.

Many thanks!

Best wishes,

Thank you for confirming this.

Your credit note ID is x.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one ...

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Thank you very much for your reply. Well, I am focusing on mouse tissue, so ab126616 is not suitable for me. And there is no sense to have a replacement from the same batch. I hope to get the refund if it is possible.

Hope this make sense.

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Thank you for your reply.

We can certainly provide you with a credit note, which can be used for a refund if the invoice has already been paid.

Sorry, in my last email I havegotten confused on the tissue you are interested in. We do ha...

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Hi there,

I followed your suggestion and did the western again. This time I used 5% BSA as blocking reagent and primary antibody dilution reagent. Other conditions I kept the same. It did not help lot. After a longer time exposure I did see sev...

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Thank you for your email. As x is not in the office this week, I will try to help to solve the problem.
I am sorry to hear that the antibody did not work as expected and that the suggestions did not improve the results.

The details provide...

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Product code: 74122
Lot number: GR67788-1
Inquiry: Hi there, I bought your product ab74122 three months ago for ME-2 WB.
I tied it in my samples which were mitochondrial fractions from tissue and did not get any band at its predicted Mw (65...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for mo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"