Anti-MEC2 antibody (ab22181)

Product name

Anti-MEC2 antibody

Description

Mouse polyclonal to MEC2

Tested applications

WBmore details

Cross reactivity

Reacts with

Caenorhabditis elegans

Immunogen

Fusion protein: GSVKVETRLVSNERSSSIQQEGAMLPSSSSKDDDLLSTSS DEVENMATRTLQQLEESTSIISANSDDDSVKKEKQAEKDV EKGNGKEEKANIQNEFGVCG, corresponding to amino acids 19/118 of Caenorhabditis elegans MEC2

GSVKVETRLV SNERSSSIQQ EGAMLPSSSS KDDDLLSTSS DEVENMATRT LQQLEESTSI ISANSDDDSV KKEKQAEKDV EKGNGKEEKA NIQNEFGVCG

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Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Constituents: 50% Glycerol

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Sensory System >> Somatosensory system >> Touch
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Cytoskeletal