Anti-TORC1 antibody [1B5] (ab104841)

Overview

• Product nameAnti-TORC1 antibody [1B5]See all TORC1 primary antibodies ...
• Description
  Mouse monoclonal [1B5] to TORC1
• Tested applicationsWB, ICC/IF, Flow Cyt, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified recombinant fragment of Human MECT1 expressed in E. coli.

• Positive controlMECT1-hIgGFc transfected HEK293 cell lysate; U251, NTERA2 and K562 cells.

Properties

• FormLiquid
• Storage instructionsStore at 4°C. Aliquot and store at -20°C long-term.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number1B5
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis

  • Signal Transduction
  • Second Messenger
  • Nucleotide Messenger
  • cAMP

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Polymerase

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionTranscriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons.
• Tissue specificityHighly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
• Involvement in diseaseNote=A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
• Sequence similaritiesBelongs to the TORC family.
• Post-translational
  modificationsPhosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (By similarity). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels.

Target information above from: UniProt accession Q6UUV9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23373 Human
  • Entrez Gene: 23373 Human
  • Omim: 607536 Human
  • SwissProt: Q6UUV9 Human
  • SwissProt: Q6UUV9 Human
  • Unigene: 371096 Human
  • Unigene: 428214 Human

• Alternative names
  KIAA0616 antibodyCREB regulated transcription coactivator 1 antibodyCREB-regulated transcription coactivator 1 antibody

  CRTC1 antibodyCRTC1_HUMAN antibodyFLJ14027 antibodyKIAA0616 antibodyMECT 1 antibodyMucoepidermoid carcinoma translocated 1 antibodyMucoepidermoid carcinoma translocated protein 1 antibodyTORC-1 antibodyTORC1 antibodyTransducer of CREB protein 1 antibodyTransducer of regulated cAMP response element binding protein 1 antibodyTransducer of regulated cAMP response element-binding protein (CREB) 1 antibodyTransducer of regulated cAMP response element-binding protein 1 antibodyWAMTP1 antibody

  see all

Anti-TORC1 antibody [1B5] images

• 

  Western blot - MECT1 antibody [1B5] (ab104841)
  Anti-TORC1 antibody [1B5] (ab104841) at 1/500 dilution + MECT1-hIgGFc transfected HEK293 cell lysate
  
  Predicted band size : 67 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - MECT1 antibody [1B5] (ab104841)
  ab104841, at 1/200 dilution, staining MECT1 in U251 (left) and NTERA2 (right) cells by Immunofluorescence (green). Actin filaments have been labeled with DY-554 phalloidin (red) and DNA with DRAQ5 fluorescent dye (blue).
• 

  Flow Cytometry - MECT1 antibody [1B5] (ab104841)
  ab104841 staining MECT1 in K562 cells (green) by Flow cytometry. The negative control is purple.