Anti-TORC1 antibody [EPR3382] (ab92477)

Overview

• Product nameAnti-TORC1 antibody [EPR3382]See all TORC1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3382] to TORC1
• Tested applicationsWB, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues in Human MECT1.

• Positive controlFetal brain and C6 cell lysates
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3382
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial Biogenesis

  • Signal Transduction
  • Second Messenger
  • Nucleotide Messenger
  • cAMP

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Polymerase

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionTranscriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons.
• Tissue specificityHighly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
• Involvement in diseaseNote=A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
• Sequence similaritiesBelongs to the TORC family.
• Post-translational
  modificationsPhosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
• Cellular localizationCytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (By similarity). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels.

Target information above from: UniProt accession Q6UUV9 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23373 Human
  • Entrez Gene: 23373 Human
  • Entrez Gene: 382056 Mouse
  • Entrez Gene: 684527 Rat
  • Entrez Gene: 684527 Rat
  • Omim: 607536 Human
  • SwissProt: Q6UUV9 Human
  • SwissProt: Q6UUV9 Human

  • SwissProt: Q68ED7 Mouse
  • SwissProt: Q157S1 Rat
  • SwissProt: Q157S1 Rat
  • Unigene: 371096 Human
  • Unigene: 428214 Human
  • Unigene: 227767 Mouse
  • Unigene: 208248 Rat

  see all

• Alternative names
  KIAA0616 antibodyCREB regulated transcription coactivator 1 antibodyCREB-regulated transcription coactivator 1 antibody

  CRTC1 antibodyCRTC1_HUMAN antibodyFLJ14027 antibodyKIAA0616 antibodyMECT 1 antibodyMucoepidermoid carcinoma translocated 1 antibodyMucoepidermoid carcinoma translocated protein 1 antibodyTORC-1 antibodyTORC1 antibodyTransducer of CREB protein 1 antibodyTransducer of regulated cAMP response element binding protein 1 antibodyTransducer of regulated cAMP response element-binding protein (CREB) 1 antibodyTransducer of regulated cAMP response element-binding protein 1 antibodyWAMTP1 antibody

  see all

Anti-TORC1 antibody [EPR3382] images

• 

  Western blot - MECT1 antibody [EPR3382] (ab92477)
  All lanes : Anti-TORC1 antibody [EPR3382] (ab92477) at 1/1000 dilution
  
  Lane 1 : Fetal brain lysate
  Lane 2 : C6 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit antibody at 1/2000 dilution
  
  Predicted band size : 67 kDa
  Observed band size : 78 kDa (why is the actual band size different from the predicted?)