Overview

  • Product name
    Anti-MED12L antibody
  • Description
    Rabbit polyclonal to MED12L
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LSVTASTRPR SPVGENADEH YSKDHDVKME IFSPMPGESC ENANTSLGRR MSVNCEKLVK REKPRELIFP SNYDLL, corresponding to amino acids 627-702 of Human MED12L

  • Positive control
    • Human liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab122368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Sequence similarities
    Belongs to the Mediator complex subunit 12 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MD12L_HUMAN antibody
    • MED12L antibody
    • Mediator complex subunit 12-like protein antibody
    • Mediator of RNA polymerase II transcription subunit 12-like protein antibody
    • NOPAR antibody
    • Thyroid hormone receptor-associated-like protein antibody
    • TNRC11L antibody
    • TRALP antibody
    • TRALPUSH antibody
    • Trinucleotide repeat-containing gene 11 protein-like antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122368 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122368, at 1/100 dilution, staining MED12L in paraffin-embedded Human liver tissue by immunohistochemistry.

References

ab122368 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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