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All lanes : Anti-MED18 antibody (ab71556) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Predicted band size : 24 kDa
Exposure time : 3 minutes
MED18 antibody (ab71556) at 3 µg/mg lysate, staining human MED18 in HeLa whole cell lysate by immunoprecipitation. 1 mg of HeLa whole cell lysate was used, 20% of IP loaded. Detection was by chemiluminescence with 30 second exposure.
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