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Products:Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Overview
Product name
Anti-MED18 antibody
See all MED18 products (4) ...
Description
Rabbit polyclonal to MED18
Tested applications
IP, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A region between residue 150 and the C-terminus of human Mediator of RNA Polymerase II Transcription, subunit 18.
Epitope
The epitope recognized by MED18 antibody (ab71556) maps to a region between residue 150 and the C-terminus (residue 208) of human MED18 using the numbering given in entry NP_060108.1 (GeneID 54797).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
IP: 2 - 5 µg/mg lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similarities
Belongs to the Mediator complex subunit 18 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9BUE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Human Mediator of RNA Polymerase II Transcription antibody
- FLJ20045 antibody
- hMED 18 antibody
see all
Database links
- Entrez Gene: 54797 Human
- Entrez Gene: 67219 Mouse
- Omim: 612384 Human
- SwissProt: Q9BUE0 Human
- SwissProt: Q9CZ82 Mouse
- Unigene: 479911 Human
- Unigene: 45929 Mouse
Anti-MED18 antibody images:
Western blot - MED18 antibody (ab71556)
All lanes : Anti-MED18 antibody (ab71556) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Predicted band size : 24 kDa
Exposure time : 3 minutes
Immunoprecipitation - MED18 antibody (ab71556)
MED18 antibody (ab71556) at 3 µg/mg lysate, staining human MED18 in HeLa whole cell lysate by immunoprecipitation. 1 mg of HeLa whole cell lysate was used, 20% of IP loaded. Detection was by chemiluminescence with 30 second exposure.
References for Anti-MED18 antibody (ab71556)
ab71556 has not yet been referenced specifically in any publications.
Publishing research using ab71556? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"