|
|
|
|
Western blot - MED18 antibody (ab74937)
(enlarge)
|
|
|
|
|
Product Name
|
MED18 antibody
|
| |
See all MED18 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to MED18
|
|
Immunogen
|
Synthetic peptide derived from an internal region of human MED18.
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/40000.
WB: 1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Extracts from Jurkat and COLO cells.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Mediator Complex
|
|
Relevance
|
MED18 is a component of the multisubunit mediator complex which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MED18 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for MED18 antibody images, references, products related to ab74937 and other tools.
|
| |
MED18 antibody images:
|
 Western blot - MED18 antibody (ab74937)
All lanes : MED18 antibody (ab74937) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from COLO cells
Lane 3 : extracts from
COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
|
| |
| |
|
Search PubMed (MEDLINE) for references to MED18
|
| |
MED18 antibody - more information
|
Customer reviews (feedback) regarding MED18 antibody |
Customer FAQs regarding MED18 antibody |
Protocols for MED18 antibody |
Price and availability of products related to MED18 antibody |
| |
MED18 antibody - related products:
|
|
|
| |
MED18 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about MED18 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
