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Products:Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Overview
Product name
Anti-MED18 antibody
See all MED18 products (4) ...
Description
Rabbit polyclonal to MED18
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from an internal region of human MED18.
Positive control
Extracts from Jurkat and COLO cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
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Western blot - MED18 antibody (ab74937)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74937 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similarities
Belongs to the Mediator complex subunit 18 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9BUE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Human Mediator of RNA Polymerase II Transcription antibody
- FLJ20045 antibody
- hMED 18 antibody
see all
Database links
- Entrez Gene: 54797 Human
- Entrez Gene: 67219 Mouse
- Omim: 612384 Human
- SwissProt: Q9BUE0 Human
- SwissProt: Q9CZ82 Mouse
- Unigene: 479911 Human
- Unigene: 45929 Mouse
Anti-MED18 antibody images:
Western blot - MED18 antibody (ab74937)
All lanes : Anti-MED18 antibody (ab74937) at 1/500 dilution
Lane 1 : extracts from Jurkat cells
Lane 2 : extracts from COLO cells
Lane 3 : extracts from
COLO cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 24 kDa
Observed band size : 24 kDa
Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MED18 antibody (ab74937)
ab74937 has not yet been referenced specifically in any publications.
Publishing research using ab74937? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"