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Products:Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Overview
Product name
Anti-MED18 antibody
See all MED18 products (4) ...
Description
Rabbit polyclonal to MED18
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide, corresponding to a region within the C terminal amino acids 150-208 of Human MED18, NP_060108.1
Positive control
Human prostate adenocarcinoma. Mouse squamous cell carcinoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
IHC-P
Application notes
IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similarities
Belongs to the Mediator complex subunit 18 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9BUE0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Human Mediator of RNA Polymerase II Transcription antibody
- FLJ20045 antibody
- hMED 18 antibody
see all
Database links
- Entrez Gene: 54797 Human
- Entrez Gene: 67219 Mouse
- Omim: 612384 Human
- SwissProt: Q9BUE0 Human
- SwissProt: Q9CZ82 Mouse
- Unigene: 479911 Human
- Unigene: 45929 Mouse
Anti-MED18 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MED18 antibody (ab84431)
Detection of Human MED18 by Immunohistochemistry.Sample: FFPE section of Human prostate adenocarcinoma.Antibody: ab84431 used at a dilution of 1/250.Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MED18 antibody (ab84431)
Detection of Mouse MED18 by Immunohistochemistry.Sample: FFPE section of Mouse squamous cell carcinoma.Antibody: ab84431 used at a dilution of 1/250.Detection: DAB staining.
References for Anti-MED18 antibody (ab84431)
ab84431 has not yet been referenced specifically in any publications.
Publishing research using ab84431? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"