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Products:Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
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Proteins and Peptides
ab58444
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Secondary antibodies
Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Publishing research using ab49271? Please let us know so that we can cite the reference in this datasheet
ab49271 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - MED19 antibody (ab49271)
ICC/IF image of ab49271 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49271, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4% PFA at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Anti-MED19 antibody (ab49271)
All lanes : Anti-MED19 antibody (ab49271) at 1 µg
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
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