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Products:Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
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Proteins and Peptides
ab58444
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Secondary antibodies
Buying a fast track?
We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.
What is a fast track? »Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab49271 for help.
There are no answered questions for ab49271
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - MED19 antibody (ab49271)
ICC/IF image of ab49271 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49271, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4% PFA at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Anti-MED19 antibody (ab49271)
All lanes : Anti-MED19 antibody (ab49271) at 1 µg
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
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