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Products:Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-MED19 antibody
See all MED19 products (2) ...
Description
Rabbit polyclonal to MED19
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human MED19.
(Peptide available as ab58444.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Mediator Complex
Applications
Application notes
IF/ICC: see image below
This antibody gave a positive result in ELISA against the immunizing peptide (ab58444).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Sequence similarities
Belongs to the Mediator complex subunit 19 family.
Cellular localization
Nucleus.
Target information above from: UniProt accessionA0JLT2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- LCMR1 antibody
- Lung cancer metastasis related protein 1 antibody
- Lung cancer metastasis-related protein 1 antibody
see all
Database links
- Entrez Gene: 219541 Human
- Entrez Gene: 381379 Mouse
- Omim: 612385 Human
- SwissProt: A0JLT2 Human
- SwissProt: Q8C1S0 Mouse
- Unigene: 43619 Human
- Unigene: 219643 Mouse
Anti-MED19 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - MED19 antibody (ab49271)
ICC/IF image of ab49271 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49271, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, Hek293 and HepG2 cells fixed in 4% PFA at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
Western blot - Anti-MED19 antibody (ab49271)
All lanes : Anti-MED19 antibody (ab49271) at 1 µg
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MED19 antibody (ab49271)
ab49271 has not yet been referenced specifically in any publications.
Publishing research using ab49271? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"