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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody (ab32866)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody (ab32866)
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Product Name 

MEF2A antibody

 

See all MEF2A antibodies (15)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MEF2A

Immunogen 

Recombinant fragment, corresponding to amino acids 272-491 of Human MEF2A

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Cross-reacts with MEF2C in EMSA

Tested applications 
(see key)

EMSA, ICC/IF, IHC-P, IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
EMSA: Use at an assay dependent dilution.
ICC/IF: 1/500.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use 4µl for 1ml cell lysate
WB: 1/500 - 1/1000. Predicted molecular weight: 55 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

 

Relevance 

MEF2A (myocyte specific enhancer factor 2) belongs to a family of DNA binding regulatory proteins. The MEF2 family of transcription factors is highly expressed in the brain when neurons undergo dendritic maturation and synapse formation. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. MEF2A also has key roles in cardiac and skeletal muscle development.

 

Alternative names MEF2A antibody (ab32866)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4205    (Human)
17258    (Mouse)
309957    (Rat)

GenBank

    

BC013437.2    (Human)

Omim

    

600660    (Human)

SwissProt

    

Q02078    (Human)
Q60929    (Mouse)
Q2MJT0    (Rat)

Unigene

    

268675    (Human)
162435    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MEF2A antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for MEF2A antibody images, references, products related to ab32866 and other tools.

 

MEF2A antibody images:

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody (ab32866)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody (ab32866)

Ab32866 staining human heart muscle. Staining is localised to the nucleus.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

 

References for MEF2A antibody (ab32866)

Specific publication references   This product has been used in: (two most recent references)

Feng H et al. Myocyte enhancer factor 2 and microphthalmia-associated transcription factor cooperate with NFATc1 to transactivate the V-ATPase d2 promoter during RANKL-induced osteoclastogenesis. J Biol Chem 284:14667-76 (2009). WB, ICC/IF; Mouse. PubMed: 19321441

Chen S et al. Transcriptional Co-activator p300 Regulates Glucose Induced Gene Expression in the Endothelial Cells. Am J Physiol Endocrinol Metab : (2009). WB; Human. PubMed: 19903865


See all 7 publication references for this product.


If you publish research using ab32866 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to MEF2A

 

MEF2A antibody - more information

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