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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody (ab32866)
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Product Name
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MEF2A antibody
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See all MEF2A antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MEF2A
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Immunogen
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Recombinant fragment, corresponding to amino acids 272-491 of Human MEF2A
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Reacts with
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Hu, Ms, Rat
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Specificity
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Cross-reacts with MEF2C in EMSA
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Tested applications
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EMSA, ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
EMSA: Use at an assay dependent dilution.
ICC/IF: 1/500.
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use 4µl for 1ml cell lysate
WB: 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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MEF2A (myocyte specific enhancer factor 2) belongs to a family of DNA binding regulatory proteins. The MEF2 family of transcription factors is highly expressed in the brain when neurons undergo dendritic maturation and synapse formation. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. MEF2A also has key roles in cardiac and skeletal muscle development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MEF2A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MEF2A antibody images, references, products related to ab32866 and other tools.
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MEF2A antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MEF2A antibody (ab32866)
Ab32866 staining human heart muscle. Staining is localised to the nucleus.
Left panel: with primary antibody. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for MEF2A antibody (ab32866)
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This product has been used in: (two most recent references)
Feng H et al. Myocyte enhancer factor 2 and microphthalmia-associated transcription factor cooperate with NFATc1 to transactivate the V-ATPase d2 promoter during RANKL-induced osteoclastogenesis. J Biol Chem 284:14667-76 (2009). WB, ICC/IF; Mouse. PubMed: 19321441
Chen S et al. Transcriptional Co-activator p300 Regulates Glucose Induced Gene Expression in the Endothelial Cells. Am J Physiol Endocrinol Metab : (2009). WB; Human. PubMed: 19903865
See all 7 publication references for this product.
If you publish research using ab32866 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MEF2A
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MEF2A antibody - more information
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MEF2A antibody - other tools:
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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