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Western blot - MEF2A antibody (ab51153)
All lanes : Anti-MEF2A antibody (ab51153) at 1/500 dilution
Lane 1 : extracts from Hela cells treated
with 125ng/ml PMA for 30min, without immunizing peptide
Lane 2 : extracts from Hela cells treated
with 125ng/ml PMA for 30min, with immunizing peptide
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)
Imunohistochemical analysis of MEF2A expression in paraffin-embedded human breast carcinoma tissue sections using 1/50 ab51153. Left: Untreated sample. Right: sample treated with immunising peptide (negative control).
Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)
ICC/IF image of ab51153 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51153, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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