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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-MEF2A antibody
See all MEF2A products (19) ...
Description
Rabbit polyclonal to MEF2A
Specificity
ab51153 recognises endogenous levels of total MEF2A protein. It can cross-react with isoform MEFA, RSRFC4, RSRFC9, and isoform 5.
Tested applications
ICC/IF, WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic non-phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-S-P-P).
Positive control
human breast carcinoma, extracts from Hela cells treated with PMA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
WB: 1/500 - 1/1000.Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA
ELISA: 1/40000ELISA: 1/40000
IHC-P: 1/50 - 1/100.
Target
Function
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
Tissue specificity
Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
Involvement in disease
Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
Sequence similarities
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain.
Post-translational
modifications
Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02078
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADCAD 1 antibody
- ADCAD1 antibody
- MADS box transcription enhancer factor 2 polypeptide A antibody
see all
Database links
- Entrez Gene: 4205 Human
- Entrez Gene: 17258 Mouse
- Entrez Gene: 309957 Rat
- GenBank: BC013437.2 Human
- Omim: 600660 Human
- SwissProt: Q02078 Human
- SwissProt: Q60929 Mouse
- SwissProt: Q2MJT0 Rat
see all
Anti-MEF2A antibody images:
Western blot - MEF2A antibody (ab51153)
All lanes : Anti-MEF2A antibody (ab51153) at 1/500 dilution
Lane 1 : extracts from Hela cells treated
with 125ng/ml PMA for 30min, without immunizing peptide
Lane 2 : extracts from Hela cells treated
with 125ng/ml PMA for 30min, with immunizing peptide
Predicted band size : 55 kDa
Observed band size : 55 kDa
Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)
Imunohistochemical analysis of MEF2A expression in paraffin-embedded human breast carcinoma tissue sections using 1/50 ab51153. Left: Untreated sample. Right: sample treated with immunising peptide (negative control).
Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)
ICC/IF image of ab51153 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MEF2A antibody (ab51153)
ab51153 has not yet been referenced specifically in any publications.
Publishing research using ab51153? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"