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Western blot - MEF2A antibody (ab51153)
Western blot - MEF2A antibody (ab51153)
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Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)
Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)
(enlarge)


Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)
Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)
(enlarge)


Product Name 

MEF2A antibody

 

See all MEF2A antibodies (15)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MEF2A

Immunogen 

Synthetic non-phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-S-P-P).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab51153 recognises endogenous levels of total MEF2A protein. It can cross-react with isoform MEFA, RSRFC4, RSRFC9, and isoform 5.

Tested applications 
(see key)

ELISA, ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/40000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

human breast carcinoma, extracts from Hela cells treated
with PMA

Cellular localization 

Nuclear

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

MEF2A (myocyte specific enhancer factor 2) belongs to a family of DNA binding regulatory proteins. The MEF2 family of transcription factors is highly expressed in the brain when neurons undergo dendritic maturation and synapse formation. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. MEF2A also has key roles in cardiac and skeletal muscle development.

 

Alternative names MEF2A antibody (ab51153)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4205    (Human)
17258    (Mouse)
309957    (Rat)

GenBank

    

BC013437.2    (Human)

Omim

    

600660    (Human)

SwissProt

    

Q02078    (Human)
Q60929    (Mouse)
Q2MJT0    (Rat)

Unigene

    

268675    (Human)
162435    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MEF2A antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for MEF2A antibody images, references, products related to ab51153 and other tools.

 

MEF2A antibody images:

Product image  Western blot - MEF2A antibody (ab51153)

Western blot - MEF2A antibody (ab51153)

All lanes : MEF2A antibody (ab51153) at 1/500 dilution

Lane 1 : extracts from Hela cells treated with 125ng/ml PMA for 30min, without immunizing peptide
Lane 2 : extracts from Hela cells treated with 125ng/ml PMA for 30min, with immunizing peptide


Predicted band size : 55 kDa
Observed band size : 55 kDa

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)

Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)

Imunohistochemical analysis of MEF2A expression in paraffin-embedded human breast carcinoma tissue sections using 1/50 ab51153. Left: Untreated sample. Right: sample treated with immunising peptide (negative control).

 

Product image  Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)

Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)

ICC/IF image of ab51153 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 
 

Search PubMed (MEDLINE) for references to MEF2A

 

MEF2A antibody - more information

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MEF2A antibody - related products:

Products for Western blot

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Lysates

 

Corresponding phospho antibody for MEF2A antibody

ab51151

  

Rabbit polyclonal to MEF2A (phospho S408)

 

Compatible Secondaries for MEF2A antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

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Alkaline Phosphatase antibody

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Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other MEF2A Antibodies

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MEF2A antibody - other tools:

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