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Western blot - MEF2A antibody (ab51153)
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Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)
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Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)
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Product Name
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MEF2A antibody
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See all MEF2A antibodies (15)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MEF2A
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Immunogen
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Synthetic non-phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-S-P-P).
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab51153 recognises endogenous levels of total MEF2A protein. It can cross-react with isoform MEFA, RSRFC4, RSRFC9, and isoform 5.
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/40000.
ICC/IF: Use at a concentration of 1 - 5 µg/ml.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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human breast carcinoma, extracts from Hela cells treated
with PMA
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Cellular localization
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Nuclear
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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MEF2A (myocyte specific enhancer factor 2) belongs to a family of DNA binding regulatory proteins. The MEF2 family of transcription factors is highly expressed in the brain when neurons undergo dendritic maturation and synapse formation. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. MEF2A also has key roles in cardiac and skeletal muscle development.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MEF2A antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MEF2A antibody images, references, products related to ab51153 and other tools.
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MEF2A antibody images:
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 Western blot - MEF2A antibody (ab51153)
All lanes : MEF2A antibody (ab51153) at 1/500 dilution
Lane 1 : extracts from Hela cells treated
with 125ng/ml PMA for 30min, without immunizing peptide
Lane 2 : extracts from Hela cells treated
with 125ng/ml PMA for 30min, with immunizing peptide
Predicted band size : 55 kDa
Observed band size : 55 kDa
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Immunohistochemistry (Paraffin-embedded sections) - MEF2A antibody (ab51153)
Imunohistochemical analysis of MEF2A expression in paraffin-embedded human breast carcinoma tissue sections using 1/50 ab51153. Left: Untreated sample. Right: sample treated with immunising peptide (negative control).
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Immunocytochemistry/ Immunofluorescence-MEF2A antibody(ab51153)
ICC/IF image of ab51153 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to MEF2A
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MEF2A antibody - more information
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Customer reviews (feedback) regarding MEF2A antibody |
Customer FAQs regarding MEF2A antibody |
Protocols for MEF2A antibody |
Price and availability of products related to MEF2A antibody |
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MEF2A antibody - related products:
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MEF2A antibody - other tools:
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Contact Abcam with a Technical Enquiry about MEF2A antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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