Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] (ab78132)

Overview

• Product nameAnti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42]See all MEK1 + MEK2 primary antibodies ...
• Description
  Rabbit monoclonal [UP42] to MEK1 + MEK2 (phospho S218 + S222 + S226)
• SpecificityRecognizes phosphorylated MEK1(Ser218/222) + MEK2 (Ser222/226)
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide: DSMANSF conjugated to KLH, corresponding to amino acids 217-223 of Human MEK1 + MEK2. The immunizing sequence is highly conserved.

  Run BLAST with Run BLAST with
• Positive controlRIPA lysates from PC-12 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberUP42
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

Applications

Target

• RelevanceMitogen activated protein kinase kinase 1 + 2 (MAPKK1 + MAPKK2) are members of a protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
• Cellular localizationCytoplasmic
• Database links
  • Entrez Gene: 5605 Human
  • Entrez Gene: 5604 Human
  • Entrez Gene: 26396 Mouse
  • Entrez Gene: 26395 Mouse
  • Entrez Gene: 170851 Rat
  • Entrez Gene: 58960 Rat
  • Omim: 601263 Human
  • Omim: 176872 Human

  • SwissProt: P36507 Human
  • SwissProt: Q02750 Human
  • SwissProt: Q63932 Mouse
  • SwissProt: P31938 Mouse
  • SwissProt: P36506 Rat
  • SwissProt: Q01986 Rat

  see all

• Alternative names
  AA589381 antibodyDual specificity mitogen activated protein kinase kinase 1 antibodyDual specificity mitogen activated protein kinase kinase 2 antibody

  Dual specificity mitogen-activated protein kinase kinase 1 antibodyDual specificity mitogen-activated protein kinase kinase 2 antibodyEC 2.7.12.2 antibodyERK activator kinase 1 antibodyERK activator kinase 2 antibodyFLJ26075 antibodyMAP kinase kinase 1 antibodyMAP kinase kinase 2 antibodyMAP2K 2 antibodyMAP2K1 antibodyMAP2K2 antibodyMAPK / ERK kinase 2 antibodyMAPK/ERK kinase 1 antibodyMAPK/ERK kinase 2 antibodyMAPKK 1 antibodyMAPKK 2 antibodyMAPKK1 antibodyMAPKK2 antibodyMEK 1 antibodyMEK 2 antibodyMEKK1 antibodyMitogen Activated Protein Kinase 1 and 2 antibodyMitogen activated protein kinase kinase 1 antibodyMitogen activated protein kinase kinase 2 antibodyMitogen activated protein kinase kinase 2 p45 antibodyMitogen-activated protein kinase kinase 2, p45 antibodyMK2 antibodyMKK 1 antibodyMKK 2 antibodyMKK1 antibodyMKK2 antibodyPRKMK 1 antibodyPRKMK 2 antibodyPRKMK1 antibodyPRKMK2 antibodyProtein kinase mitogen activated kinase 1 antibodyprotein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibodyProtein kinase, mitogen-activated, kinase 1 antibodyProtein kinase, mitogen-activated, kinase 2 antibody

  see all

Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] images

• 

  Western blot - MEK1 + MEK2 (phospho S218 + S222) antibody [Not given] (ab78132)
  All lanes : Anti-MEK1 + MEK2 (phospho S218 + S222 + S226) antibody [UP42] (ab78132) at 1/5000 dilution
  
  Lane 1 : PC-12 cell lysate
  Lane 2 : PC-12 cell lysate treated with 5 Units of B-Raf for 30 minutes at 30°C.
  
  Secondary
  HRP-goat anti-rabbit secondary at 1/5000 dilution
  
  Predicted band size : 44 kDa
  Observed band size : 50 kDa (why is the actual band size different from the predicted?)