Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (ab54494)
Overview
- Product nameAnti-MEK1 + MEK2 (phospho S218 + S222) antibodySee all MEK1 + MEK2 primary antibodies ...
- DescriptionRabbit polyclonal to MEK1 + MEK2 (phospho S218 + S222)
- Specificityab54494 recognizes the ~45 kDa MEK1 and MEK2 proteins phosphorylated at Ser218 and Ser222.
- Tested applicationsELISA, Dot Blot, WB more details
- Species reactivityReacts with: Human
- Immunogen
A synthetic phosphopeptide corresponding to amino acids surrounding the Ser218 and Ser222 phosphorylation sites of human MEK1 + MEK2.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab54494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| Dot Blot | |
| WB |
ELISA: 1/1000.
WB: 1/1000. Predicted molecular weight: 43/44 kDa. May also detect a higher molecular weight band.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- RelevanceMitogen activated protein kinase kinase 1 + 2 (MAPKK1 + MAPKK2) are members of a protein kinase family that activate the ERK 1 + 2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop. MEK 1 + 2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
- Cellular localizationCytoplasmic
-
Database links
- Entrez Gene: 5605 Human
- Entrez Gene: 5604 Human
- Omim: 601263 Human
- Omim: 176872 Human
- SwissProt: P36507 Human
- SwissProt: Q02750 Human
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Alternative names
- AA589381 antibodyDual specificity mitogen activated protein kinase kinase 2 antibodyDual specificity mitogen-activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 2 antibodyEC 2.7.12.2 antibodyERK activator kinase 1 antibodyERK activator kinase 2 antibodyFLJ26075 antibodyMAP kinase kinase 1 antibodyMAP kinase kinase 2 antibodyMAP2K 2 antibodyMAP2K1 antibodyMAP2K2 antibodyMAPK / ERK kinase 2 antibodyMAPK/ERK kinase 1 antibodyMAPK/ERK kinase 2 antibodyMAPKK 1 antibodyMAPKK 2 antibodyMAPKK1 antibodyMAPKK2 antibodyMEKK1 antibodyMitogen Activated Protein Kinase 1 and 2 antibodyMitogen activated protein kinase kinase 1 antibodyMitogen activated protein kinase kinase 2 antibodyMitogen activated protein kinase kinase 2 p45 antibodyMitogen-activated protein kinase kinase 2, p45 antibodyMK2 antibodyMKK 1 antibodyMKK 2 antibodyMKK1 antibodyMKK2 antibodyPRKMK 1 antibodyPRKMK 2 antibodyPrkmk1 antibodyPrkmk2 antibodyprotein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibodyProtein kinase, mitogen-activated, kinase 1 antibodyProtein kinase, mitogen-activated, kinase 2 antibody
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References for Anti-MEK1 + MEK2 (phospho S218 + S222) antibody (ab54494)
ab54494 has not yet been referenced specifically in any publications.
