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- Secondary antibodies
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Product name
Anti-MEK1 (phospho T386) antibody
See all MEK1 products (29) ...
Description
Rabbit polyclonal to MEK1 (phospho T386)
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
a wide range of other species
Immunogen
Phosphopeptide derived from a region of human MEK 1 that contains threonine 386.
Positive control
NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free) as a carrier. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 1. The final product is generated by affinity chromatography using a MEK 1 derived peptide that is phosphorylated at threonine 386.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
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Western blot - MEK1 (phospho T386) antibody (ab5615)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 50 kDa.
Target
Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Tissue specificity
Widely expressed, with extremely low levels in brain.
Involvement in disease
Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Target information above from: UniProt accessionQ02750
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 1 antibody
- Dual specificity mitogen-activated protein kinase kinase 1 antibody
see all
Database links
- Entrez Gene: 5604 Human
- Entrez Gene: 26395 Mouse
- Entrez Gene: 170851 Rat
- Omim: 176872 Human
- SwissProt: Q02750 Human
- SwissProt: P31938 Mouse
- SwissProt: Q01986 Rat
- Unigene: 145442 Human
see all
Anti-MEK1 (phospho T386) antibody images:
Western blot - MEK1 (phospho T386) antibody (ab5615)
Peptide Competition: Extracts prepared from NIH3T3 cells left untreated (lane 1) or treated (lanes 2-5) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab5615 antibody for two hours at room temperature, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), the non-phosphopeptide corresponding to the immunogen (3), or, generic phosphothreonine containing peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5615 blocks the antibody signal, but the corresponding non-phosphopeptide does not, thereby demonstrating the specificity of the antibody. The data also show the induction of the ab5615 phospho signal upon stimulation with PDGF (compare lanes 1 and 5).
References for Anti-MEK1 (phospho T386) antibody (ab5615)
ab5615 has not yet been referenced specifically in any publications.
Publishing research using ab5615? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"