Overview

  • Product nameAnti-MEK2 antibody [1A1]
    See all MEK2 primary antibodies
  • Description
    Mouse monoclonal [1A1] to MEK2
  • Tested applicationsIP, WBmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Recombinant human protein purified from E.coli (His-MEK2)

  • Positive control
    • MCF-7, U937, Jurkat T and K562 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesIP: Use 2µl for 400µl cell lysates.
    WB: 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
    • Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
      Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiofaciocutaneous syndrome antibody
      • CFC syndrome antibody
      • CFC4 antibody
      • Dual specificity mitogen activated protein kinase kinase 2 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 2 antibody
      • MAP2K2 antibody
      • MAPK / ERK kinase 2 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK 2 antibody
      • MAPKK2 antibody
      • MEK 2 antibody
      • MEK2 antibody
      • Microtubule associated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 p45 antibody
      • MKK 2 antibody
      • MKK2 antibody
      • MP2K2_HUMAN antibody
      • OTTHUMP00000165826 antibody
      • OTTHUMP00000165827 antibody
      • PRKMK 2 antibody
      • PRKMK2 antibody
      see all

    Anti-MEK2 antibody [1A1] images

    • All lanes : Anti-MEK2 antibody [1A1] (ab70590) at 1/2000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : Jurkat T cell lysate
      Lane 4 : K562 cell lysate


      Predicted band size : 44 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)

    References for Anti-MEK2 antibody [1A1] (ab70590)

    ab70590 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70590.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"