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  • Product nameAnti-MEK2 antibody [1A1]
    See all MEK2 primary antibodies
  • Description
    Mouse monoclonal [1A1] to MEK2
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant human protein purified from E.coli (His-MEK2)

  • Positive control
    • MCF-7, U937, Jurkat T and K562 cell lysates.



Our Abpromise guarantee covers the use of ab70590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).


  • FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiofaciocutaneous syndrome antibody
    • CFC syndrome antibody
    • CFC4 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 2 antibody
    • map2k2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK 2 antibody
    • MAPKK2 antibody
    • MEK 2 antibody
    • MEK2 antibody
    • Microtubule associated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • MKK 2 antibody
    • MKK2 antibody
    • MP2K2_HUMAN antibody
    • OTTHUMP00000165826 antibody
    • OTTHUMP00000165827 antibody
    • PRKMK 2 antibody
    • PRKMK2 antibody
    see all

Anti-MEK2 antibody [1A1] images

  • Predicted band size : 44 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MEK2 knockout HAP1 cell lysate (20 µg)

    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab70590 observed at 44 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab70590 was shown to recognize MEK2 when MEK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab70590 and ab181602 (loading control to GAPDH) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-MEK2 antibody [1A1] (ab70590) at 1/2000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : Jurkat T cell lysate
    Lane 4 : K562 cell lysate

    Predicted band size : 44 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

References for Anti-MEK2 antibody [1A1] (ab70590)

ab70590 has not yet been referenced specifically in any publications.

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