Overview

  • Product nameAnti-MEK2 antibody [7F5]See all MEK2 primary antibodies ...
  • Description
    Mouse monoclonal [7F5] to MEK2
  • Tested applicationsWB, ELISA, ICC/IF, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-400 of Human MEK2

  • Positive control
    • PC-12, Jurkat, Hela and NIH/3T3 cell lysates. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab93924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
ICC/IF
Flow Cyt
  • Application notesELISA: 1/10000.
    Flow Cyt: Use at an assay dependent dilution.
    ICC/IF: Use at an assay dependent dilution.
    WB: 1/1000 - 1/2000. Predicted molecular weight: 44 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
    • Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
      Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Target information above from: UniProt accession P36507 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Cardiofaciocutaneous syndrome antibody
      • CFC syndrome antibody
      • CFC4 antibody
      • Dual specificity mitogen activated protein kinase kinase 2 antibody
      • Dual specificity mitogen-activated protein kinase kinase 2 antibody
      • ERK activator kinase 2 antibody
      • FLJ26075 antibody
      • MAP kinase kinase 2 antibody
      • MAP2K2 antibody
      • MAPK / ERK kinase 2 antibody
      • MAPK/ERK kinase 2 antibody
      • MAPKK 2 antibody
      • MAPKK2 antibody
      • MEK 2 antibody
      • MEK2 antibody
      • Microtubule associated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 antibody
      • Mitogen activated protein kinase kinase 2 p45 antibody
      • MKK 2 antibody
      • MKK2 antibody
      • MP2K2_HUMAN antibody
      • OTTHUMP00000165826 antibody
      • OTTHUMP00000165827 antibody
      • PRKMK 2 antibody
      • PRKMK2 antibody
      see all

    Anti-MEK2 antibody [7F5] images

    • All lanes : Anti-MEK2 antibody [7F5] (ab93924) at 1/1000 dilution

      Lane 1 : PC-12 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : NIH/3T3 cell lysate


      Predicted band size : 44 kDa
    • ab93924 staining MEK2 in HeLa cells at 100-400 dilution by Immunofluorescence (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye is blue.
    • ab93924 staining MEK2 in HeLa cells by Flow cytometry (green) at 100-400 dilution. The negative control is purple.

    References for Anti-MEK2 antibody [7F5] (ab93924)

    ab93924 has not yet been referenced specifically in any publications.

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