The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
Use at an assay dependent concentration.
1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. Contains 1 protein kinase domain.
Post-translational modificationsMAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Dual specificity mitogen activated protein kinase kinase 2 antibody
Dual specificity mitogen-activated protein kinase kinase 2 antibody
ERK activator kinase 2 antibody
MAP kinase kinase 2 antibody
MAPK / ERK kinase 2 antibody
MAPK/ERK kinase 2 antibody
MAPKK 2 antibody
MEK 2 antibody
Microtubule associated protein kinase kinase 2 antibody
Mitogen activated protein kinase kinase 2 antibody
Mitogen activated protein kinase kinase 2 p45 antibody
MKK 2 antibody
PRKMK 2 antibody
Anti-MEK2 antibody [Y78] images
Western blot - Anti-MEK2 antibody [Y78] (ab32517)
Predicted band size : 44 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: MEK2 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg) Lane 4: K562 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab32517 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa. ab32517 was shown to specifically react with MEK2 when MEK2 knockout samples were used. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab32517 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
ab32517 staining MEK2 in wild-type HAP1 cells (top panel) and MEK2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32517 at 1μg/ml and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin embedded prostate
carcinoma using ab32517 at a dilution of 1/250.Immunohistochemical analysis of paraffin embedded prostate
carcinoma using ab32517 at a dilution of 1/250.
Flow Cytometry - MEK2 antibody [Y78] (ab32517)
Overlay histogram showing HeLa cells stained with ab32517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32517, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-MEK2 antibody [Y78] (ab32517)
This product has been referenced in:
Li L et al. MEK1 promotes YAP and their interaction is critical for tumorigenesis in liver cancer. FEBS Lett587:3921-7 (2013).
Read more (PubMed: 24211253) »
Yamashita S et al. The Leydig cell MEK/ERK pathway is critical for maintaining a functional population of adult Leydig cells and for fertility. Mol Endocrinol25:1211-22 (2011).
Read more (PubMed: 21527500) »