Anti-MEK2 antibody [Y78] (ab32517)

Overview

• Product nameAnti-MEK2 antibody [Y78]See all MEK2 primary antibodies ...
• Description
  Rabbit monoclonal [Y78] to MEK2
• SpecificityThis antibody does not cross react with other MAP kinase kinase family members
• Tested applicationsICC/IF, WB, IHC-P, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  Synthetic peptide corresponding to residues in N terminus of human MEK2

• Positive controlJurkat cell lysate, HeLa cells, prostate carcinoma
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• Clone numberY78
• IsotypeIgG
• Research Areas
  • Cancer
  • Signal transduction
  • Protein phosphorylation
  • Serine/threonine kinases
  • MAPK pathway

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Other

Applications

Target

• FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
• Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
• Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsMAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
  Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Target information above from: UniProt accession P36507 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5605 Human
  • Entrez Gene: 407835 Human
  • Entrez Gene: 26396 Mouse
  • Omim: 601263 Human
  • SwissProt: P36507 Human
  • SwissProt: Q63932 Mouse
  • Unigene: 465627 Human
  • Unigene: 275436 Mouse

• Alternative names
  Cardiofaciocutaneous syndrome antibodyCFC syndrome antibodyDual specificity mitogen activated protein kinase kinase 2 antibody

  Dual specificity mitogen-activated protein kinase kinase 2 antibodyERK activator kinase 2 antibodyFLJ26075 antibodyMAP kinase kinase 2 antibodyMAP2K 2 antibodymap2k2 antibodyMAPK / ERK kinase 2 antibodyMAPK/ERK kinase 2 antibodyMAPKK 2 antibodyMAPKK2 antibodyMEK 2 antibodyMEK2 antibodyMicrotubule Associated Protein Kinase Kinase 2 antibodyMitogen activated protein kinase kinase 2 antibodyMitogen activated protein kinase kinase 2 p45 antibodyMKK 2 antibodyMKK2 antibodyMP2K2_HUMAN antibodyOTTHUMP00000165826 antibodyOTTHUMP00000165827 antibodyPRKMK 2 antibodyPRKMK2 antibody

  see all

Anti-MEK2 antibody [Y78] images

• 

  Western blot - MEK2 antibody [Y78] (ab32517)
  Anti-MEK2 antibody [Y78] (ab32517) at 1/10000 dilution + Jurkat cell lysate
  
  Predicted band size : 44 kDa
  Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - MEK2 antibody [Y78] (ab32517)
  Immunofluorescent staining of HeLa cells using ab32517 at a dilution of 1/250.
• 

  Immunohistochemistry (Paraffin-embedded sections) - MEK2 antibody [Y78] (ab32517)
  Immunohistochemical analysis of paraffin embedded prostate carcinoma using ab32517 at a dilution of 1/250.
• 

  Flow Cytometry - MEK2 antibody [Y78] (ab32517)
  Overlay histogram showing HeLa cells stained with ab32517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32517, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.