Overview

  • Product nameAnti-MEK2 (phospho T394) antibody
    See all MEK2 primary antibodies
  • Description
    Rabbit polyclonal to MEK2 (phospho T394)
  • Specificityab30622 recognises endogenous levels of MEK2 only when phosphorylated at Threonine 394.
  • Tested applicationsWB, ELISA, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human MEK2 around the phosphorylation site of Threonine 394.

  • Positive control
    • ovary cancer cells, breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab30622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • Involvement in diseaseDefects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiofaciocutaneous syndrome antibody
    • CFC syndrome antibody
    • CFC4 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • ERK activator kinase 2 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 2 antibody
    • MAP kinase kinase 2 antibody
    • MAP2K 2 antibody
    • MAP2K 2 antibody
    • map2k2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK 2 antibody
    • MAPKK 2 antibody
    • MAPKK2 antibody
    • MAPKK2 antibody
    • MEK 2 antibody
    • MEK2 antibody
    • Microtubule Associated Protein Kinase Kinase 2 antibody
    • Microtubule Associated Protein Kinase Kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • MKK 2 antibody
    • MKK 2 antibody
    • MKK2 antibody
    • MKK2 antibody
    • MP2K2_HUMAN antibody
    • OTTHUMP00000165826 antibody
    • OTTHUMP00000165827 antibody
    • PRKMK 2 antibody
    • PRKMK2 antibody
    see all

Anti-MEK2 (phospho T394) antibody images

  • All lanes : Anti-MEK2 (phospho T394) antibody (ab30622) at 1/500 dilution

    Lane 1 : extracts from ovary cancer cells.
    Lane 2 : extracts from ovary cancer cells, preincubated with synthesized non phosphopeptide
    Lane 3 : extracts from ovary cancer cells, preincubated with synthesized phosphopeptide (negative control).


    Predicted band size : 44 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 MEK2 (Phospho Thr394) Antibody (ab30622). Left: untreated sample; Right: sample preincubated with synthesized phosphopeptide (negative control).
  • ICC/IF image of ab30622 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30622, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MEK2 (phospho T394) antibody (ab30622)

This product has been referenced in:
  • Fan AC  et al. Nanofluidic proteomic assay for serial analysis of oncoprotein activation in clinical specimens. Nat Med 15:566-71 (2009). Read more (PubMed: 19363496) »

See 1 Publication for this product

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Thank you for your enquiry. Due to my limited access to journals, I was not able to determine which specific publication indicates the negative feedback. However, I have included below all the references that we have on this product. I hope one of t...

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