Overview

  • Product nameAnti-MEK3 antibody [1D10]See all MEK3 primary antibodies ...
  • Description
    Mouse monoclonal [1D10] to MEK3
  • Tested applicationsSandwich ELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human MEK3 protein.

  • Positive control
    • Human Placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab117633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionDual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • Tissue specificityAbundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • Involvement in diseaseNote=Defects in MAP2K3 may be involved in colon cancer.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Target information above from: UniProt accession P46734 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Anti-MEK3 antibody [1D10] images

  • ab117633, at 5 µg/ml, staining MEK3 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.
  • sELISA showing the detection limit for recombinant proprietary tagged MEK3 protein is approximately 0.03ng/ml as a capture antibody (ab117633).

References for Anti-MEK3 antibody [1D10] (ab117633)

ab117633 has not yet been referenced specifically in any publications.

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