Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)

Overview

  • Product name
    Anti-MEK4 (phospho S80) antibody [EP1075Y]
    See all MEK4 primary antibodies
  • Description
    Rabbit monoclonal [EP1075Y] to MEK4 (phospho S80)
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminal of human MKK4

  • Positive control
    • 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP 1/50.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
    • Tissue specificity
      Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    • Information by UniProt
    • Database links
    • Alternative names
      • c Jun N terminal kinase kinase 1 antibody
      • C-JUN N-terminal kinase kinase 1 antibody
      • Dual specificity mitogen activated protein kinase kinase 4 antibody
      • Dual specificity mitogen-activated protein kinase kinase 4 antibody
      • JNK Activated Kinase 1 antibody
      • JNK activating kinase 1 antibody
      • JNK-activating kinase 1 antibody
      • JNKK antibody
      • JNKK1 antibody
      • MAP kinase kinase 4 antibody
      • Map2k4 antibody
      • MAPK ERK kinase 4 antibody
      • MAPK/ERK kinase 4 antibody
      • MAPKK 4 antibody
      • MAPKK4 antibody
      • MEK 4 antibody
      • MEK4 antibody
      • Mitogen activated protein kinase kinase 4 antibody
      • MKK 4 antibody
      • MKK4 antibody
      • MP2K4_HUMAN antibody
      • PRKMK4 antibody
      • SAPK ERK kinase 1 antibody
      • SAPK/ERK kinase 1 antibody
      • SAPKK 1 antibody
      • SAPKK1 antibody
      • SEK1 antibody
      • SERK1 antibody
      • SKK1 antibody
      • Stress activated protein kinase kinase 1 antibody
      see all

    Images

    • ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
    • All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution

      Lane 1 : 3T3 cell lysate untreated
      Lane 2 : 3T3 cell lysate treated with PDGF

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    References

    ab52958 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (10)
    Sample
    Human Cell lysate - whole cell (HepG2)
    Specification
    HepG2
    Treatment
    EGF 50ng/ml for 2hrs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up