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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-MEK6 (phospho S207) antibody
See all MEK6 products (12) ...
Description
Rabbit polyclonal to MEK6 (phospho S207)
Specificity
This antibody detects endogenous levels of MKK6 only when phosphorylated at Serine207.
Tested applications
WB, ELISA, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic phosphopeptide derived from human MKK6 around the phosphorylation site of Serine 207 (V-D-SP-V-A).
Positive control
Breast carcinoma tissue and MDA-MB-435 cell extracts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 37 kDa.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Tissue specificity
Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Weakly autophosphorylated.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Target information above from: UniProt accessionP52564
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Dual specificity mitogen activated protein kinase kinase 6 antibody
- MKK 6 antibody
- Dual specificity mitogen activated protein kinase kinase 6 antibody
see all
Database links
- Entrez Gene: 5608 Human
- Entrez Gene: 114495 Rat
- Omim: 601254 Human
- SwissProt: P52564 Human
- SwissProt: Q925D6 Rat
- Unigene: 463978 Human
- Unigene: 17256 Rat
Anti-MEK6 (phospho S207) antibody images:
Immunohistochemistry (Paraffin-embedded sections) - MKK6 (phospho S207) antibody (ab36722)
Immunohistochemical analysis of paraffin embedded breast carcinoma, using ab36772 diluted 1:50. Left: Untreated Ab; Right: Ab pre-treated with synthesized phosphopeptide.
Western blot - MKK6 (phospho S207) antibody (ab36722)
All lanes : Anti-MEK6 (phospho S207) antibody (ab36722) at 1/500 dilution
Lane 1 : Extracts from untreated MDA-MB-435 cells
Lane 2 : Extracts from UV treated MDA-MB-435 cells
Lysates/proteins at 30 µg per lane.
Predicted band size : 37 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
UV treatment protocol:
a. For adherent cells, cells were plated on 100-mm culture dishes with growth medium (basic medium containing 10% serum).
b. When the cells were at the log phase and 80-90% confluent, the culture media was drained. Cells were washed with fresh basic media (no serum) once.
c. Add 3ml fresh basic media per dish before irradiation.
d. Culture dishes were uncovered in a UV cross-linker. UV irradiation was carried out with 100 J/m2 (or with various other dosages).
e. Cells can be directly used to prepare cell lysis. If needed to reculture in some experiments, 3ml growth medium was added to per dish, and the cells were incubated at 37°C for 1~2 hours in a CO2 incubator.
For suspension cells, cells were collected by centrifugation (1000g, 10min) from cell supernatant from flasks. Resuspend the cells in 3ml of fresh basic media, and plate on a 100-mm plate for irradiation.
Immunocytochemistry/ Immunofluorescence-MEK6 (phospho S207) antibody(ab36722)
ICC/IF image of ab36722 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36722, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MEK6 (phospho S207) antibody (ab36722)
ab36722 has not yet been referenced specifically in any publications.
Publishing research using ab36722? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"