Anti-MAP3K1 antibody [2F6] - BSA and Azide free (ab212601)
Key features and details
- Mouse monoclonal [2F6] to MAP3K1 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MAP3K1 antibody [2F6] - BSA and Azide free
See all MAP3K1 primary antibodies -
Description
Mouse monoclonal [2F6] to MAP3K1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human MAP3K1 aa 1050-1200.
Database link: Q13233 -
Positive control
- IHC: Human uterine carcinoma tissue, human thyroid carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab212601 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
2F6 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
for 30 minutes at RT |
Notes |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. for 30 minutes at RT |
Target
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Function
Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 RING-type zinc finger.
Contains 1 SWIM-type zinc finger. -
Post-translational
modificationsAutophosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 4214 Human
- Entrez Gene: 26401 Mouse
- Entrez Gene: 116667 Rat
- Omim: 600982 Human
- SwissProt: Q13233 Human
- SwissProt: P53349 Mouse
- SwissProt: Q62925 Rat
- Unigene: 653654 Human
see all -
Alternative names
- M3K1_HUMAN antibody
- MAP3K1 antibody
- MAPK/ERK kinase kinase 1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterine carcinoma tissue labeling MAP3K1 with ab212601 at 1 μg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid carcinoma tissue labeling MAP3K1 with ab212601 at 1 μg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling MAP3K1 with ab212601 at 1 μg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab212601 has been referenced in 1 publication.
- Liu L et al. An RFC4/Notch1 signaling feedback loop promotes NSCLC metastasis and stemness. Nat Commun 12:2693 (2021). PubMed: 33976158