Overview
- Product nameAnti-MEKK2 antibodySee all MEKK2 primary antibodies ...
- DescriptionRabbit polyclonal to MEKK2
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse - Immunogen
Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 13-43 of Mouse MEKK2.
- Positive control293 cell lysates, transiently transfected with the MEKK2 gene.
Properties
- FormLiquid
- Storage instructionsStore at 4°C (up to 6 months). For long term storage store at -20°C
- Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab116348 is purified through a Protein A column, followed by peptide affinity purification.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab116348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/100 - 1/500. Predicted molecular weight: 70 kDa. |
Target
- FunctionComponent of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
- Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain. - Post-translational
modificationsAutophosphorylated. - Cellular localizationCytoplasm. Nucleus. Upon EGF stimulation, translocates into the nucleus.
-
Database links
- Entrez Gene: 10746 Human
- Entrez Gene: 10746 Human
- Entrez Gene: 26405 Mouse
- Omim: 609487 Human
- SwissProt: Q9Y2U5 Human
- SwissProt: Q9Y2U5 Human
- SwissProt: Q61083 Mouse
- Unigene: 145605 Human
- Unigene: 211762 Mouse
see all
Target information above from: UniProt accession
Q9Y2U5
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- M3K2_HUMAN antibodyMap3k2 antibodyMAPK/ERK kinase kinase 2 antibody
- MEK kinase 2 antibodyMEKK 2 antibodyMEKK2b antibodyMitogen activated protein kinase kinase kinase 2 antibodyMitogen-activated protein kinase kinase kinase 2 antibody
see all
Anti-MEKK2 antibody images
-
Western blot - Anti-MEKK2 antibody (ab116348)All lanes : Anti-MEKK2 antibody (ab116348) at 1/100 dilution
Lane 1 : 293 cell lysates, non-transfected
Lane 2 : 293 cell lysates, MEKK2 transfected
Lysates/proteins at 2 µg per lane.
Predicted band size : 70 kDa
References for Anti-MEKK2 antibody (ab116348)
ab116348 has not yet been referenced specifically in any publications.
