Anti-MELK antibody [2G2] (ab129373)

Overview

• Product nameAnti-MELK antibody [2G2]See all MELK primary antibodies ...
• Description
  Mouse monoclonal [2G2] to MELK
• Tested applicationsELISA, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
• Immunogen

  Synthetic peptide: C-VYKRLVEDILSSCKV (with a Cysteine residue linker), corresponding to C terminal amino acids 637-651 of Human MELK (Q14680).

  Run BLAST with Run BLAST with
• Positive controlHepG2 cells and MCF-7 cells. Human liver cancer tissue.

Properties

• FormLiquid
• Storage instructionsStore at 4°C or at -20°C for long term storage.
• Storage bufferPreservative: 0.05% Sodium azide
  Constituent: 99% PBS
• Concentration information loading...
• PurityIgG fraction
• Purification notesab129373 is purified.
• Clonality Monoclonal
• Clone number2G2
• IsotypeIgG2a
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

Applications

Target

• FunctionPhosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
• Tissue specificityExpressed in placenta, kidney, thymus, testis, ovary and intestine.
• Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
  Contains 1 KA1 (kinase-associated) domain.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsAutophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q14680 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 520088 Cow
  • Entrez Gene: 9833 Human
  • Entrez Gene: 9833 Human
  • Entrez Gene: 17279 Mouse
  • Omim: 607025 Human
  • SwissProt: Q14680 Human
  • SwissProt: Q14680 Human
  • SwissProt: Q61846 Mouse

  • Unigene: 184339 Human
  • Unigene: 268668 Mouse

  see all

• Alternative names
  AI327312 antibodyhMELK antibodyHPK 38 antibody

  hPK38 antibodyKIAA0175 antibodyLikely ortholog of maternal embryonic leucine zipper kinase antibodyMaternal embryonic leucine zipper kinase antibodyMELK antibodyMELK_HUMAN antibodymKIAA0175 antibodyMPK38 antibodyOTTHUMP00000021377 antibodyOTTHUMP00000046113 antibodypEg3 kinase antibodyProtein kinase Eg3 antibodyProtein kinase PK38 antibodyRP23 382O11.1 antibodyTyrosine protein kinase MELK antibody

  see all

Anti-MELK antibody [2G2] images

• 

  Flow Cytometry - Anti-MELK antibody [2G2] (ab129373)
  ab129373 at 1/200 dilution (green) or negative control (red) staining MELK in MCF-7 cells by flow cytometry.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-MELK antibody [2G2] (ab129373)
  ab129373 at 1/200 dilution staining MELK (green) in HepG2 cells by immunofluorescence. DNA is stained with DRAQ fluorescent dye (blue) and actin filaments with Alexa Fluor-555 phalloidin (red).
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
  ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MELK antibody [2G2] (ab129373)
  ab129373 at 1/200 dilution staining MELK in paraffin-embedded liver cancer tissue by immunohistochemistry, with DAB staining.