Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-MELK antibody - C-terminal
See all MELK products (5) ...
Description
Rabbit polyclonal to MELK - C-terminal
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Positive control
HEPG2 tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab71287 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
-
Western blot - MELK antibody - C-terminal (ab71287)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Target
Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ14680
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
see all
Database links
- Entrez Gene: 9833 Human
- Omim: 607025 Human
- SwissProt: Q14680 Human
- Unigene: 184339 Human
Anti-MELK antibody - C-terminal images:
Western blot - MELK antibody - C-terminal (ab71287)
Anti-MELK antibody - C-terminal (ab71287) at 1/100 dilution + HEPG2 tissue lysate at 12.5 µg
Predicted band size : 75 kDa
Observed band size : 75 kDa
Additional bands at : 20 kDa,50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MELK antibody - C-terminal (ab71287)
ab71287 has not yet been referenced specifically in any publications.
Publishing research using ab71287? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"