|
|
|
|
Western blot - MELK antibody - C-terminal (ab71287)
(enlarge)
|
|
|
|
|
Product Name
|
MELK antibody - C-terminal
|
| |
See all MELK antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to MELK - C-terminal
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HEPG2 tissue lysate.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
|
|
Relevance
|
MELK, maternal embryonic leucine zipper kinase, belongs to the protein kinase superfamily. It contains 1 protein kinase domain and is expressed in placenta, kidney, thymus, testis, ovary and intestine.
MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may also be involved in the inhibition of spliceosome assembly during mitosis.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Protein G purified
|
|
Storage buffer
|
Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
ab71287 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|
|
Form
|
Liquid
|
|
Concentration
|
0.250 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MELK antibody - C-terminal is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for MELK antibody - C-terminal images, references, products related to ab71287 and other tools.
|
| |
MELK antibody - C-terminal images:
|
 Western blot - MELK antibody - C-terminal (ab71287)
MELK antibody - C-terminal (ab71287) at 1/100 dilution + HEPG2 tissue lysate at 12.5 µg
Predicted band size : 75 kDa
Observed band size : 75 kDa
Additional bands at : 20 kDa,50 kDa. We are unsure as to the identity of these extra bands.
|
| |
| |
|
Search PubMed (MEDLINE) for references to MELK
|
| |
MELK antibody - C-terminal - more information
|
Customer reviews (feedback) regarding MELK antibody - C-terminal |
Customer FAQs regarding MELK antibody - C-terminal |
Protocols for MELK antibody - C-terminal |
Price and availability of products related to MELK antibody - C-terminal |
| |
MELK antibody - C-terminal - related products:
|
|
|
| |
MELK antibody - C-terminal - other tools:
|
Contact Abcam with a Technical Enquiry about MELK antibody - C-terminal |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
