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Read our guarantee »Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Anti-MELK antibody - C-terminal
See all MELK products (5) ...
Rabbit polyclonal to MELK - C-terminal
ELISA, WBmore details
Reacts with
Human
HEPG2 tissue lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration information loading...
Protein G purified
ab71287 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Polyclonal
IgG
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Western blot - MELK antibody - C-terminal (ab71287)
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Our Abpromise guarantee covers the use of ab71287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000
WB: 1/100 - 1/500.Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Expressed in placenta, kidney, thymus, testis, ovary and intestine.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
Cytoplasm.
Target information above from: UniProt accessionQ14680
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MELK antibody - C-terminal (ab71287)

Anti-MELK antibody - C-terminal (ab71287) at 1/100 dilution + HEPG2 tissue lysate at 12.5 µg
Predicted band size : 75 kDa
Observed band size : 75 kDa
Additional bands at : 20 kDa,50 kDa. We are unsure as to the identity of these extra bands.
ab71287 has not yet been referenced specifically in any publications.
Publishing research using ab71287? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
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