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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-MELK antibody
See all MELK products (5) ...
Description
Rabbit polyclonal to MELK
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide derived from an internal sequence of human MELK.
Positive control
K562 cell extract.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Western blot - MELK antibody (ab72703)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ14680
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
see all
Database links
- Entrez Gene: 9833 Human
- Omim: 607025 Human
- SwissProt: Q14680 Human
- Unigene: 184339 Human
Anti-MELK antibody images:
Western blot - MELK antibody (ab72703)
All lanes : Anti-MELK antibody (ab72703) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 75 kDa
Observed band size : 75 kDa
Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.
References for Anti-MELK antibody (ab72703)
ab72703 has not yet been referenced specifically in any publications.
Publishing research using ab72703? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"