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Western blot - MELK antibody (ab72703)
(enlarge)
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Product Name
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MELK antibody
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See all MELK antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MELK
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Immunogen
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Synthetic peptide derived from an internal sequence of human MELK.
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Reacts with
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Hu
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
WB: 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K562 cell extract.
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Cellular localization
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Cytoplasmic
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Relevance
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MELK, maternal embryonic leucine zipper kinase, belongs to the protein kinase superfamily. It contains 1 protein kinase domain and is expressed in placenta, kidney, thymus, testis, ovary and intestine.
MELK phosphorylates ZNF622 and may contribute to its redirection to the nucleus. It may also be involved in the inhibition of spliceosome assembly during mitosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MELK antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MELK antibody images, references, products related to ab72703 and other tools.
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MELK antibody images:
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 Western blot - MELK antibody (ab72703)
All lanes : MELK antibody (ab72703) at 1/500 dilution
Lane 1 : K562 cell extract
Lane 2 : K562 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 75 kDa
Observed band size : 75 kDa
Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to MELK
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MELK antibody - more information
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Customer reviews (feedback) regarding MELK antibody |
Customer FAQs regarding MELK antibody |
Protocols for MELK antibody |
Price and availability of products related to MELK antibody |
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MELK antibody - related products:
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MELK antibody - other tools:
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Contact Abcam with a Technical Enquiry about MELK antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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