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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Overview
Product name
Anti-MELK antibody
See all MELK products (5) ...
Description
Goat polyclonal to MELK
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: KRLVEDILSSCKV, corresponding to C terminal amino acids 639-651 of Human MELK.
KRLVEDILSS CKV
Positive control
Jurkat and HeLa cell lysates
General notes
Principal Names - MELK, maternal embryonic leucine zipper kinase, KIAA0175 kinase; KIAA0175: likely ortholog of maternal embryonic leucine zipper kinase
GenBank Accession Number – NP_055606
LocusLink ID - 9833
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Stem Cells >> Embryonic Stem Cells >> Intracellular
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
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Western blot - MELK antibody (ab9696)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 0.5 - 2 µg/ml. Approx 120-150kDa band seen in Jurkat and HeLa cell lysates. Please note that the band we consistently detect in western blots differs from the predicted size of 72kDa. Complete blocking by the immunising peptide is observed with peptide neutralisation experiments. It is to be noted that MELK is a member of AMPK family, which has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press]. Further the activity of this antibody was confirmed against recombinant fusion MELK protein by Western blotting. Use RIPA lysis buffer.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
Tissue specificity
Expressed in placenta, kidney, thymus, testis, ovary and intestine.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 KA1 (kinase-associated) domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ14680
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI327312 antibody
- hMELK antibody
- HPK 38 antibody
see all
Database links
- Entrez Gene: 9833 Human
- Omim: 607025 Human
- SwissProt: Q14680 Human
- Unigene: 184339 Human
Anti-MELK antibody images:
Western blot - MELK antibody (ab9696)
Predicted band size : 72 kDa
ab9696 (0.5µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
References for Anti-MELK antibody (ab9696)
This product has been referenced in:
- Lin MLet al. Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family. Breast Cancer Res 9:R17 (2007).Read more (PubMed: 17280616) »
See 1 publication for this product
Publishing research using ab9696? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"