Anti-MGEA5 antibody (ab105217)

Overview

• Product nameAnti-MGEA5 antibodySee all MGEA5 primary antibodies ...
• Description
  Rabbit polyclonal to MGEA5
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rabbit, Horse, Cow, Dog, Pig, Macaque Monkey, Gorilla
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human MGEA5.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: U87mg; JEG3; PDX1.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

Applications

Target

• FunctionCleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3.
• Tissue specificityUbiquitous. Shows highest expression in the brain, placenta and pancreas.
• Post-translational
  modificationsProteolytically cleaved by caspase-3.
• Cellular localizationCytoplasm and Nucleus.

Target information above from: UniProt accession O60502 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 10724 Human
  • Entrez Gene: 10724 Human
  • Entrez Gene: 10724 Human
  • Entrez Gene: 76055 Mouse
  • Omim: 604039 Human
  • SwissProt: O60502 Human
  • SwissProt: O60502 Human
  • SwissProt: O60502 Human

  • SwissProt: Q9EQQ9 Mouse
  • Unigene: 500842 Human
  • Unigene: 122725 Mouse
  • Unigene: 440640 Mouse
  • Unigene: 480399 Mouse

  see all

• Alternative names
  Beta-N-acetylhexosaminidase antibodyBifunctional protein NCOAT antibodyGlycoside hydrolase O-GlcNAcase antibody

  HAT antibodyHEXC antibodyHexosaminidase C antibodyHistone acetyltransferase antibodyHyaluronidase in meningioma antibodyKIAA0679 antibodyMEA5 antibodyMeningioma expressed antigen 5 antibodyMeningioma-expressed antigen 5 antibodyMGEA 5 antibodyMgea5 antibodyN-acetyl-beta-D-glucosaminidase antibodyN-acetyl-beta-glucosaminidase antibodyNCOAT antibodyNCOAT_HUMAN antibodyNuclear cytoplasmic O GlcNAcase and acetyltransferase antibodyNuclear cytoplasmic O-GlcNAcase and acetyltransferase antibodyO-GlcNAcase antibodyOGA antibody

  see all

Anti-MGEA5 antibody images

• 

  Western blot - Anti-MGEA5 antibody (ab105217)
  All lanes : Anti-MGEA5 antibody (ab105217) at 1 µg/ml
  
  Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
  Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
  Lane 3 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate
  Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Immunising peptide at 1 µg/ml
  Lane 5 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
  Lane 6 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate with Immunising peptide at 1 µg/ml
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 103 kDa
  Observed band size : 120 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 4 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-MGEA5 antibody (ab105217)

  ab105217 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105217 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.